FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822945

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822945
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11995
Sequences flagged as poor quality0
Sequence length151
%GC60

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCACGGTACAATCG4563.8015839933305546No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCACGGTACAATCT2722.2676115047936642No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC2622.1842434347644852No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1871.558982909545644No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACGCACGGTACAATCG1010.8420175072947061No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCACGGTACAAT940.7836598582742809No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCACGGTAC790.6586077532305127No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGCAC610.5085452271779909No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG560.46686119216340144No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA550.45852438516048355No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC460.3834931221342226No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT410.3418090871196332No Hit
ATTGCGCAATGCAATGCATGGGGGCCAACCGGGAGAAAATGACCCAGATT360.30012505210504375No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG340.28345143809920803No Hit
ATTGCGCAATGGTCGCGCCGGGCGGTCGCAAGATTAAAACTCAAAGGAAT340.28345143809920803No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACGCACGGTACAATCT330.27511463109629014No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT320.2667778240933722No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT310.25844101709045436No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT260.2167569820758649No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA260.2167569820758649No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCACGGTACAAGCT260.2167569820758649No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCACGGTACAAGCG260.2167569820758649No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGCA210.17507294706127552No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG210.17507294706127552No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC210.17507294706127552No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCAC210.17507294706127552No Hit
ATTGCGCAATGTGTGTGCCGGGGGAATTGACGGGGGCCTGCACAAGCGGT200.16673614005835766No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT200.16673614005835766No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC200.16673614005835766No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACGCACGGTA200.16673614005835766No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC190.15839933305543977No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC180.15006252605252188No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG180.15006252605252188No Hit
ATTGCGCAATGGGCGTAAAGGGTGCGTAGGCGGGTCTTTAAGTCAGGGGT170.14172571904960402No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT170.14172571904960402No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG170.14172571904960402No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC160.1333889120466861No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCACGGTACAATGG160.1333889120466861No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG160.1333889120466861No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGCACGGTACAATCGCG150.12505210504376824No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCACG140.11671529804085036No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG140.11671529804085036No Hit
GGGGGCTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG130.10837849103793246No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCACGGTACACTCG130.10837849103793246No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT120.1000416840350146No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT120.1000416840350146No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGGC120.1000416840350146No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT120.1000416840350146No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)