FastQCFastQC Report
Tue 7 May 2024
EGAF00007822956

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822956
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5394
Total Bases814.4 kbp
Sequences flagged as poor quality0
Sequence length151
%GC59

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT4177.7308120133481655No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC2063.819058212829069No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC430.7971820541342233No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.5376344086021506No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.5376344086021506No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC240.44493882091212456No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG190.3522432332220986No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAG180.33370411568409347No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG180.33370411568409347No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGGTGGGGGT160.29662588060808304No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC150.27808676307007785No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGGGGGGGGT150.27808676307007785No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGGTTGTGTT140.25954764553207266No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA140.25954764553207266No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC140.25954764553207266No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA130.2410085279940675No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG130.2410085279940675No Hit
CATCTGACGCTGCCGACGAGGAAGTGGTTGTGTAGATCTCGGTGGTCGCC110.2039302929180571Illumina Single End PCR Primer 1 (95% over 22bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC110.2039302929180571No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.2039302929180571No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG100.18539117538005193No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA100.18539117538005193No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATAT100.18539117538005193No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGGTGGGGTT90.16685205784204674No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGGTG90.16685205784204674No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGGTTGTGTA90.16685205784204674No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGGTGGTGTT90.16685205784204674No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGGGGGGGGG90.16685205784204674No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA90.16685205784204674No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAG90.16685205784204674No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG90.16685205784204674No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.16685205784204674No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA80.14831294030404152No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGGTGGTGGT80.14831294030404152No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA80.14831294030404152No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAA70.12977382276603633No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGGGG70.12977382276603633No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGGTT70.12977382276603633No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGGGTGTGTT70.12977382276603633No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGGGTGTGGT70.12977382276603633No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA70.12977382276603633No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGGGGGGGTT70.12977382276603633No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAAA70.12977382276603633No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGGTTGTGGT70.12977382276603633No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA70.12977382276603633No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAAA60.11123470522803114No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATATGT60.11123470522803114No Hit
TCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA60.11123470522803114No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAA60.11123470522803114No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60.11123470522803114No Hit
GTATAAGAGACAGATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.11123470522803114No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)