FastQCFastQC Report
Tue 7 May 2024
EGAF00007822958

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822958
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4246
Total Bases641.1 kbp
Sequences flagged as poor quality0
Sequence length151
%GC60

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT3448.101742816768724No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1353.1794630240226094No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC290.6829957607159679No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC260.6123410268487989No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG220.5181347150259068No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGGGGGGGGT220.5181347150259068No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGGTGGGGGT190.4474799811587376No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.40037682524729157No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG160.3768252472915685No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.30617051342439944No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG130.30617051342439944No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA130.30617051342439944No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC120.2826189354686764No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGGTTGTGTA120.2826189354686764No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA120.2826189354686764No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG110.2590673575129534No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAG110.2590673575129534No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGGGGGTGTT100.23551577955723035No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGGTTGTGTT90.2119642016015073No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAA80.18841262364578426No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA80.18841262364578426No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC80.18841262364578426No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGGGGGTGGG70.16486104569006124No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC70.16486104569006124No Hit
CATCTGACGCTGCCGACGAGGAAGTGGTTGTGTAGATCTCGGTGGTCGCC60.1413094677343382Illumina Single End PCR Primer 1 (95% over 22bp)
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGGTT60.1413094677343382No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGGTGGTGTT60.1413094677343382No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGGGGGGGGG60.1413094677343382No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGGGGGTGGT60.1413094677343382No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGGGTGGGGT50.11775788977861518No Hit
CATCTGACGCTGCCGACGAGGAAGTGGGGGGGGGGGTGGGGGGGGGGGGG50.11775788977861518No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGGTGGGGTT50.11775788977861518No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGGGTGTGTT50.11775788977861518No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC50.11775788977861518No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA50.11775788977861518No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG50.11775788977861518No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCCA50.11775788977861518No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAAT50.11775788977861518No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)