FastQCFastQC Report
Tue 7 May 2024
EGAF00007822964

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822964
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2919
Total Bases440.7 kbp
Sequences flagged as poor quality0
Sequence length151
%GC63

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT37712.915381980130181No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1505.138746145940391No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC341.1647824597464886No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.41109969167523125No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG110.3768413840356286No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC90.3083247687564234No Hit
CATCTGACGCTGCCGACGAGGAAGTGGTTGTGTAGATCTCGGTGGTCGCC80.2740664611168208Illumina Single End PCR Primer 1 (95% over 22bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.2740664611168208No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG70.2398081534772182No Hit
GCAGAAGTGCCGAGCTCTACCAGCCATGGAACCCTATCTCCGAACAAATC70.2398081534772182No Hit
CATCTGACGCTGCCGACGAGGAAGTGGGGGGGGTGGTGGGGGGGGGGGGG70.2398081534772182No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAA60.20554984583761562No Hit
GCTCTACCAGCCATGGAACCCTATCTCCGAACAAATCGATTTCAGGGTGA60.20554984583761562No Hit
CATCTGACGCTGCCGACGAGGAAGTGGGGGGGGTGTTGTGGGGGGGGGGG60.20554984583761562No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGGTT60.20554984583761562No Hit
GTGTATAAGAGACCGATTGCGCAATCTGTCTCTTATACACATCTGACGCT50.17129153819801302No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG50.17129153819801302No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA50.17129153819801302No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.17129153819801302No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAG40.1370332305584104No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAAT40.1370332305584104No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGGGG40.1370332305584104No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGGTGGGGTT40.1370332305584104No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA40.1370332305584104No Hit
CATCTGACGCTGCCGACGAGGAAGTGGGGGGGGTGGTGTGGGGGGGGGGG30.10277492291880781No Hit
CTACCAGCCATGGAACCCTATCTCCGAACAAATCGATTTCAGGGTGACAG30.10277492291880781No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGAAAA30.10277492291880781No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAA30.10277492291880781No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGAAGTGGGGGGGGGGGTGGG30.10277492291880781No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCC30.10277492291880781No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG30.10277492291880781No Hit
CATCTGACGCTGCCGACGAGGAAGTGGGGGGGGGGGTGGGGGGGGGGGGG30.10277492291880781No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAG30.10277492291880781No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC30.10277492291880781No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA30.10277492291880781No Hit
CATCTGACGCTGCCGACGAGGAAGTGGGGGGGGGGTTGTGGGGGGGGGGG30.10277492291880781No Hit
CTCTTTCCAGGGCCCTCGCCGACGTCTCCTGGTTCGTTTACGTTGCCGTC30.10277492291880781No Hit
CATCTGACGCTGCCGACGAGGAAGTGGGGGGGGGTTTGTGGGGGGGGGGG30.10277492291880781No Hit
CTTATACACATCTGACGCTGCCGACGAGGAAGTGGGGGGGGGGGTGGGGG30.10277492291880781No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGAAA30.10277492291880781No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGGGTGTGTT30.10277492291880781No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA30.10277492291880781No Hit
GTGTCTTTCACACTGTTTGTCGTTACTCATGCCAGCATTCGCACTTCTGA30.10277492291880781No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.10277492291880781No Hit
CATCTGACGCTGCCGACGAGGAAGTGGGGGGGGGGGTGTGGGGGGGGGGG30.10277492291880781No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA30.10277492291880781No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC30.10277492291880781No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAATATGT30.10277492291880781No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)