FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822970

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822970
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17446
Sequences flagged as poor quality0
Sequence length151
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCAGGTAGGGTGTA7674.396423248882265No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT6043.4621116588329706No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC3702.1208299896824485No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCAGGTAGGGTGTT2111.2094462914135047No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCAGGTAGGGTGGA1891.083342886621575No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCAGGTAGGGTG1020.5846612403989453No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCAGGTAGG1000.5731972945087699No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCAGGTAGGGTGGT830.4757537544422791No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCAG650.37257824143070045No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT550.31525851197982346No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC530.30379456608964805No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC440.25220680958385877No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG410.23501089074859566No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG360.20635102602315716No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG340.19488708013298178No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG340.19488708013298178No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA330.18915510718789408No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT320.18342313424280637No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320.18342313424280637No Hit
CTTATACACATCTGACGCTGCCGACGATTCAGGTAGGGTGTAGATCTCGG320.18342313424280637No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCAGGTAG290.1662272154075433No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA280.16049524246245558No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC280.16049524246245558No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCA250.14329932362719247No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAAT230.1318353777370171No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC230.1318353777370171No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC220.12610340479192939No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA210.12037143184684168No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA210.12037143184684168No Hit
GTATAAGAGACAGATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.10890748595666629No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA190.10890748595666629No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCAGGGAGGGTGTA180.10317551301157858No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT180.10317551301157858No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)