FastQCFastQC Report
Tue 7 May 2024
EGAF00007822974

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822974
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1987
Total Bases300 kbp
Sequences flagged as poor quality0
Sequence length151
%GC65

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT25812.984398590840463No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC844.227478610971314No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC271.3588324106693508No Hit
CATCTGACGCTGCCGACGAGGAAGTGGTTGTGTAGATCTCGGTGGTCGCC90.45294413688978363Illumina Single End PCR Primer 1 (95% over 22bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC60.3019627579265224No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA50.25163563160543534No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG40.20130850528434827No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA40.20130850528434827No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG40.20130850528434827No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.1509813789632612No Hit
CATCTGACGCTGCCGACGAGGAAGTGGGGGGGGTGGTGTGGGGGGGGGGG30.1509813789632612No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA30.1509813789632612No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAG30.1509813789632612No Hit
CATCTGACGCTGCCGACGAGGAAGTGGGGGTGGTGTTGTGGGGGGGGGGG30.1509813789632612No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGGGG30.1509813789632612No Hit
CATCTGACGCTGCCGACGAGGAAGTGGGGGGGGTGTTGGGGGGGGGGGGG30.1509813789632612No Hit
CATCTGACGCTGCCGACGAGGAAGTGGGGGGGGTGTTGTGGGGGGGGGGG30.1509813789632612No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG30.1509813789632612No Hit
CATCTGACGCTGCCGACGAGGAAGTGGTGGTGGGGTTGGGGGGGGGGGGG30.1509813789632612No Hit
CATCTGACGCTGCCGACGAGGAAGTGGTTGTGGTGTTGTGGGGGGGGGGG30.1509813789632612No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGGTGGGGGT30.1509813789632612No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC30.1509813789632612No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGA30.1509813789632612No Hit
TCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGAAA20.10065425264217413No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGGTTGTGGT20.10065425264217413No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAAAATGT20.10065425264217413No Hit
GACACAGTACTCCTGGAAGTCAACTTCATTGTCCCTGTTGCTGTCCAAGT20.10065425264217413No Hit
CATCTGACGCTGCCGACGAGGAAGTGGGGGGGGTGGTTTGGGGGGGGGGG20.10065425264217413No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAATATGT20.10065425264217413No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGAAGTGGTGGTGGTGTTGGG20.10065425264217413No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT20.10065425264217413No Hit
CATCTGACGCTGCCGACGAGGAAGTGGTTGTGGTGTGGTGGGGGGGGGGG20.10065425264217413No Hit
CATCTGACGCTGCCGACGAGGAAGTGGGGGTGTTGTTGTGGGGGGGGGGG20.10065425264217413No Hit
CATCTGACGCTGCCGACGAGGAAGTGGTGGGGTTGGTGTGGGTGGGGGGG20.10065425264217413No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAATA20.10065425264217413No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAAT20.10065425264217413No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGGGGGGGGT20.10065425264217413No Hit
GGAGTAGGAGAGAGGGAGGTAAGAGTCAGAAGCTTATGTTGTTTATGCGG20.10065425264217413No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC20.10065425264217413No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATAT20.10065425264217413No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAATA20.10065425264217413No Hit
CATCTGACGCTGCCGACGAGGAAGTGGGGGGGGTGTGTTGGGGGGGGGGG20.10065425264217413No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAA20.10065425264217413No Hit
CATCTGACGCTGCCGACGAGGAAGTGGTGGGGTTGTTGTGGGGGGGGGGG20.10065425264217413No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATCT20.10065425264217413No Hit
CTCCTTATCTGGGCAGCCCTCAAAGAATTCATTGCACATCATGGCAATGC20.10065425264217413No Hit
CATCTGACGCTGCCGACGAGGAAGTGGTTGGGGTTTTTTGGGGGGGGGGG20.10065425264217413No Hit
CATCTGACGCTGCCGACGAGGAAGTGGGTGTGGTGTTGTGGGGGGGGGGG20.10065425264217413No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.10065425264217413No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAAAAA20.10065425264217413No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAAAAT20.10065425264217413No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATAT20.10065425264217413No Hit
CTTATACACATCTGACGCTGCCGACGAGGAAGTGGTGGTGTTGTTGTGGG20.10065425264217413No Hit
CATCTGACGCTGCCGACGAGGAAGTGGTGGGGGTGTTGTGGGGGGGGGGG20.10065425264217413No Hit
GTATAAGAGACAGATTGCGCAATGAGTTCCCAGGGGTCGCATTGCGCAAT20.10065425264217413No Hit
CCCTAGTCCTATTGGTAGCTCTACCTCCAACGACCTCAACCACGACGCTG20.10065425264217413No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAAAATTTG20.10065425264217413No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAGTGTCTC20.10065425264217413No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAA20.10065425264217413No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGAAAAA20.10065425264217413No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA20.10065425264217413No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA20.10065425264217413No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTGCGAAAA20.10065425264217413No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAAATT20.10065425264217413No Hit
CATCTGACGCTGCCGACGAGGAAGTGGGGGGGTGGGTGTGGGGGGGGGGG20.10065425264217413No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAATA20.10065425264217413No Hit
CATCTGACGCTGCCGACGAGGAAGTGGGTGGGGTGTTGTGGGGGGGGGGG20.10065425264217413No Hit
CATCTGACGCTGCCGACGAGGAAGTGGTGGGGGGGGTGGGGGGGGGGGGG20.10065425264217413No Hit
CATCTGACGCTGCCGACGAGGAAGTGGGGGGGGTTTTGTGGGGGGGGGGG20.10065425264217413No Hit
CCTCCAACGACCTCAACCACGACGCTGTACCTCAATACATTTCGGGGAGT20.10065425264217413No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAAAA20.10065425264217413No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA20.10065425264217413No Hit
CTTATACACATCTGACGCTGCCGACGAGGAAGTGGGGGTGTGGGTGGGGG20.10065425264217413No Hit
CATCTGACGCTGCCGACGAGGAAGTGGTTGTGTAGATCTAGGTGGTCGCC20.10065425264217413Illumina RNA PCR Primer (95% over 21bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCC20.10065425264217413No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAAT20.10065425264217413No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAAA20.10065425264217413No Hit
CATCTGACGCTGCCGACGAGGAAGTGGGGGGGTTGGTGTGGGGGGGGGGG20.10065425264217413No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA20.10065425264217413No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGGTGGGGGG20.10065425264217413No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATA20.10065425264217413No Hit
CTTATACACATCTGACGCTGCCGACGAGGAAGTGGTGGGGGGGGTGTGGG20.10065425264217413No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAA20.10065425264217413No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC20.10065425264217413No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGGTG20.10065425264217413No Hit
GTGTAGCCCTGGCTGTCCTGGAACTCACTCTATAGACCAGGCTGGCCTTG20.10065425264217413No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGAAATATTT20.10065425264217413No Hit
CATCTGACGCTGCCGACGAGGAAGTGGTGGTGGTGTTGGGGGGGGGGGGG20.10065425264217413No Hit
GCTAGGCAGCTCCCTGGTCAGTAGCTCCTTGAGCTCTGTCTTGTTCAGCT20.10065425264217413No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)