FastQCFastQC Report
Mon 16 Jan 2023
EGAF00007822976

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822976
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7424
Sequences flagged as poor quality0
Sequence length151
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT5036.775323275862069No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC2503.3674568965517246No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC540.7273706896551724No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAG400.5387931034482759No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGGTGGGGGT330.4445043103448276No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280.3771551724137931No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGGGGGGGGT240.3232758620689655No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG230.3098060344827586No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG220.2963362068965517No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA220.2963362068965517No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG210.2828663793103448No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGGTGGTGTT200.26939655172413796No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC190.25592672413793105No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGGTTGTGTA180.24245689655172414No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.24245689655172414No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGGTGGTGGT170.2289870689655172No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG160.21551724137931033No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA160.21551724137931033No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGGTGGGGTT150.20204741379310345No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAA140.18857758620689655No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC140.18857758620689655No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC140.18857758620689655No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGGGGGGGGG130.17510775862068967No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGGTTGTGGT130.17510775862068967No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA130.17510775862068967No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT120.16163793103448276No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGGTTGTGTT110.14816810344827586No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGGTGGGG110.14816810344827586No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAG110.14816810344827586No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGGGGGTGGT110.14816810344827586No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAAA100.13469827586206898No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGGTG100.13469827586206898No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC100.13469827586206898No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA100.13469827586206898No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA90.12122844827586207No Hit
CATCTGACGCTGCCGACGAGGAAGTGGTTGTGTAGATCTCGGTGGTCGCC90.12122844827586207Illumina Single End PCR Primer 1 (95% over 22bp)
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGGGTGTGTT90.12122844827586207No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA90.12122844827586207No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCA90.12122844827586207No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAA90.12122844827586207No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGGTTGGGGT90.12122844827586207No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGGGGGTGTT90.12122844827586207No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGGGT80.10775862068965517No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATGTGT80.10775862068965517No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGGTGGTG80.10775862068965517No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGGGTGTGGT80.10775862068965517No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGGTGGTGGG80.10775862068965517No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGGGGGGGTT80.10775862068965517No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA80.10775862068965517No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATCTGTCT80.10775862068965517No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGAA80.10775862068965517No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)