FastQCFastQC Report
Tue 7 May 2024
EGAF00007822991

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822991
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13038
Total Bases1.9 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC4463.420770056757171No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTATGGCCTTATCG4343.3287314005215523No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3532.707470470931124No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTATGGCCTTATCT2942.2549470777726643No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2822.1629084215370455No Hit
ATTGCGCAATGTGAGGTCCGGGGGCTGCCCTCCGGCCTCTGGTGAAGCCC2601.9941708851050775No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTATGGCCT720.5522319374137138No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG690.5292222733548091No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTATGGCCTTAT630.4832029452369995No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC600.46019328117809477No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC510.39116428900138056No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG420.32213529682466635No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAT390.2991256327657616No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA390.2991256327657616No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT390.2991256327657616No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT370.28378585672649176No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTTATGGCCTTATCG310.23776652860868233No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA310.23776652860868233No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA290.22242675256941247No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTATGGCCGTATCG260.19941708851050774No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG250.19174720049087282No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT240.18407731247123793No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG240.18407731247123793No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC230.176407424451603No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT220.1687375364319681No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC220.1687375364319681No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAT200.15339776039269826No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTA180.13805798435342845No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG180.13805798435342845No Hit
ATTGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.13038809633379353No Hit
GGGGGCTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG160.12271820831415861No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCT150.11504832029452369No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC140.10737843227488879No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTATGGCCTGATCG140.10737843227488879No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC140.10737843227488879No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTATG140.10737843227488879No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)