FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822992

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822992
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13038
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGGACTCGGTGTA7936.082221199570486No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT5764.41785549930971No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC3402.6077619266758707No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2071.5876668200644273No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1321.0124252185918086No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGGACTCGGGGTA1080.8283479061205707No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGGACTCG800.613591041570793No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT710.5445620493940788No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAA660.5062126092959043No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC560.42951372909955515No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG550.42184384107992023No Hit
CTTATACACATCTGACGCTGCCGACGAAACGGACTCGGTGTAGATCTCGG540.41417395306028537No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT540.41417395306028537No Hit
CTCTTATACACATCTGACGCTGCCGACGAAACGGACTCGGTGTAGATCTC520.3988341770210155No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGGACTCGGTG480.36815462494247586No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG420.32213529682466635No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGG410.3144654088050315No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC370.28378585672649176No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG330.25310630464795214No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC320.24543641662831722No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA310.23776652860868233No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA310.23776652860868233No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC290.22242675256941247No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC270.20708697653014269No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC260.19941708851050774No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGGACTCGGTGTT250.19174720049087282No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG240.18407731247123793No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGG230.176407424451603No Hit
TCTCAGCACAGTAAGATTTGTTGCACATCAGCAGCACCTCCAGCTCCTTG220.1687375364319681No Hit
CTTATACACATCTGACGCTGCCGACGAAACGGACTCGGTGTAGTTCTCGG210.16106764841233318No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA200.15339776039269826No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT200.15339776039269826No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC190.14572787237306337No Hit
CTTATACACATCTGACGCTGCCGACGAAACGGACTCGGGGTAGATCTCGG180.13805798435342845No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA180.13805798435342845No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACG180.13805798435342845No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA170.13038809633379353No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA170.13038809633379353No Hit
CATCTGACGCTGCCGACGAAACGGACTCGGTGTAGATCTCGGTGGTCGCC160.12271820831415861Illumina RNA PCR Primer (95% over 22bp)
CTCTTATACACATCTGACGCTGCCGACGAAACGGACTCGGTGTAGTTCTC160.12271820831415861No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA150.11504832029452369No Hit
ATACACATCTGACGCTGCCGACGAAACGGACTCGGTGTAGATCTCGGTGG150.11504832029452369No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA150.11504832029452369No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAA140.10737843227488879No Hit
GAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA140.10737843227488879No Hit
GTGAGCAATCTCAGCACAGTAAGATTTGTTGCACATCAGCAGCACCTCCA140.10737843227488879No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)