FastQCFastQC Report
Tue 7 May 2024
EGAF00007822993

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822993
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12574
Total Bases1.8 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGGCATCCATCG5054.0162239541911875No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGGCATCCATCT3692.934627008111977No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC2231.7735008748210594No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1941.5428662319071098No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1531.2167965643391125No Hit
ATTGCGCAATGGATACAAGGGGGAGGGTGGACGCAGTGGTTCTGCACTGA1311.0418323524733577No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGGCATC970.7714331159535549No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGGCATCCAT740.5885159853666295No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT740.5885159853666295No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC720.5726101479242882No Hit
ATTGCGCAATGAGAACGTCGGGAGGAACAAAGAAAAGGAGGGGGAAGAGA660.5248926355972642No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAG650.5169397168760935No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTTAGGCATCCATCG620.4930809607125815No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG580.46126928582789883No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT550.4374105296643868No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC500.3976459360585335No Hit
ATTGCGCAATGGCTGGGGGGGGCTGGAGCTTCGGTTACCTACCCTCAGCA470.37378717989502147No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT410.3260696675679975No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT380.30221091140448547No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG370.29425799268331476No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG330.2624463177986321No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA320.25449339907746144No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG320.25449339907746144No Hit
ATTGCGCAATGGGTTAACGGGGGAAGAAAGAGGAGGAGGAGAAGAGAAGA310.24654048035629075No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTTAGGCATCCATCT310.24654048035629075No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC310.24654048035629075No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC300.2385875616351201No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA290.23063464291394942No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC250.19882296802926674No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAG240.1908700493080961No Hit
ATTGCGCAATGTAGGGCAGGGGGAAGAAAGAGGAGGAGGAGAAGAGAAGA230.1829171305869254No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG230.1829171305869254No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA210.16701129314458407No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGGCAT210.16701129314458407No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT200.1590583744234134No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT200.1590583744234134No Hit
ATTGCGCAATGGACCTCGCGGGCGGGGAGGTGGAGCACGAGCGCACGTGT200.1590583744234134No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT200.1590583744234134No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG190.15110545570224274No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGGCATCCAGCG180.14315253698107205No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGG170.1351996182599014No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG160.12724669953873072No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC160.12724669953873072No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTA160.12724669953873072No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTG160.12724669953873072No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCT150.11929378081756005No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC150.11929378081756005No Hit
ATTGCGCAATGACGCGGCCGGGGGGAGAAGGGGGACCACTGCTGGTGACA150.11929378081756005No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGGCATCCAGCT150.11929378081756005No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGCT140.11134086209638938No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGC130.10338794337521871No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT130.10338794337521871No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT130.10338794337521871No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT130.10338794337521871No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)