FastQCFastQC Report
Tue 7 May 2024
EGAF00007822997

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822997
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13220
Total Bases1.9 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCTCGTCATCG4443.358547655068079No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC3782.859304084720121No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCTCGTCATCT3292.4886535552193645No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1931.459909228441755No Hit
ATTGCGCAATGGCCGGGTTGGGGAAGCAACAGGCACCATTCAGGAAGATT1180.8925869894099849No Hit
ATTGCGCAATGAGGTCGAGGGGGATTACGAAATATTACAGTAATAAAAAT980.7413010590015128No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA940.7110438729198185No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG910.6883509833585476No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC870.6580937972768532No Hit
ATTGCGCAATGATTGGCGTGGGGGGGAGTACGGTCGCAAGATTAAAACTC770.5824508320726172No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCTCGT700.529500756429652No Hit
ATTGCGCAATGACCGCGCGGGGGTTTCTGCAAAATCGTAAGATGAAGTAT670.5068078668683812No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCG630.47655068078668683No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT570.4311649016641452No Hit
ATTGCGCAATGAAGAGGACGGGAGGATAGAGATTGTGTGTGAGAGGGAGG570.4311649016641452No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCTCGTCAT560.4236006051437216No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT480.3630862329803328No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG480.3630862329803328No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT420.3177004538577912No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTTCGCTCGTCATCG370.27987897125567324No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGC370.27987897125567324No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC340.25718608169440244No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG330.24962178517397882No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC300.22692889561270801No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA300.22692889561270801No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG300.22692889561270801No Hit
ATTGCTCAATGAGGTCGAGGGGGATTACGAAATATTACAGTAATAAAAAT280.2118003025718608No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTC270.2042360060514372No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG270.2042360060514372No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC250.18910741301059003No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT230.17397881996974282No Hit
ATTGCGCAATGTGTCAGGTGGGCAAGGGCGCATGGTGGATGCCTTGGCAA220.1664145234493192No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC190.1437216338880484No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT190.1437216338880484No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC190.1437216338880484No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGCT180.1361573373676248No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCTCG170.12859304084720122No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG170.12859304084720122No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT160.1210287443267776No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC160.1210287443267776No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG150.11346444780635401No Hit
ATTGCGCAATGCGAGTTACGGGGGGTAGTGCCTCGTAACTAACTCTCTGG150.11346444780635401No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTG150.11346444780635401No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT150.11346444780635401No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCTCGTCATCGCG140.1059001512859304No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCTCGTCAGCG140.1059001512859304No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA140.1059001512859304No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGG140.1059001512859304No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA140.1059001512859304No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)