FastQCFastQC Report
Tue 7 May 2024
EGAF00007823013

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823013
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12576
Total Bases1.8 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC4463.546437659033079No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCTCCAGAATCG3682.926208651399491No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2902.3059796437659035No Hit
ATTGCGCAATGGAGGGGCCGGGAGAGCCCGGGAGTCAAAGCCGGTTGTCA2772.202608142493639No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2532.01176844783715No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCTCCAGAATCT2371.8845419847328244No Hit
ATTGCGCAATGAGGTTTTTGGGGATTTCCGCTTCCGGCGCGCCGGTGTAG1631.296119592875318No Hit
ATTGCGCAATGATGAACCCGGGATCGCAGCCGCCAAAATGAAGTTCAATC1160.9223918575063612No Hit
ATTGCGCAATGTGATCTCGGGGGAAGGTGGGGATGACGTCAAGTCCTCAT1130.8985368956743003No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCTCCAG1040.826972010178117No Hit
ATTGCGCAATGGGATCGCTGGGGAAGGTGGGGATGACGTCAAGTCCTCAT960.7633587786259541No Hit
ATTGCGCAATGGCGAACTGGGGGATTGATGATGTGGATGCCCCCGCAGAG890.7076972010178118No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC830.6599872773536896No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGC550.4373409669211196No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC520.4134860050890585No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG500.3975826972010178No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA480.38167938931297707No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA480.38167938931297707No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT470.37372773536895676No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG460.3657760814249364No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT410.3260178117048346No Hit
ATTGCGCAATGCAAGGGCTGGGGGGTCCCCACCAGCACAAGAAGCCGTAC410.3260178117048346No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT390.3101145038167939No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCTCCAGAAT390.3101145038167939No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT390.3101145038167939No Hit
ATTGCGCAATGATGAACCCGGGATCGTAGCCGCCAAAATGAAGTTCAATC350.2783078880407125No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTAGCTCCAGAATCG340.27035623409669207No Hit
ATTGCGCAATGGATGGTCAGGGAAGTAGGGGGTTTCAATGCTGTTGGGAA340.27035623409669207No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA310.24650127226463103No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC290.2305979643765903No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG260.20674300254452924No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGC240.19083969465648853No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG240.19083969465648853No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC230.1828880407124682No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG200.1590330788804071No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAG190.15108142493638677No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC190.15108142493638677No Hit
ATTGCGCAATGCTACTGGGGGGTCCCCACCAGCACAAGAAGCCGTACGTG180.1431297709923664No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG170.13517811704834604No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCTCCA170.13517811704834604No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTAGCTCCAGAATCT160.1272264631043257No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT150.11927480916030535No Hit
ATTGCGCAATCTGTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGA150.11927480916030535No Hit
ATTGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.11132315521628498No Hit
ATTGCGCAATGTATGCTGCTGCTGTCTCTTATACACATCTCCGAGCCCAC140.11132315521628498No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGC140.11132315521628498No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG130.10337150127226462No Hit
ATTGCGCAATGCGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAG130.10337150127226462No Hit
CCGCTACCCCGATCCCCTCATCAAGGTGAATGATACCATTCAGATTGATT130.10337150127226462No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)