FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007823014

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823014
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12576
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGGACTCGGTGTA5934.715330788804072No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT5634.47678117048346No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC3582.8466921119592876No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1501.1927480916030535No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGGACTCG1060.8428753180661578No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT980.779262086513995No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGGACTCGGGGTA870.691793893129771No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAA800.6361323155216284No Hit
CTTATACACATCTGACGCTGCCGACGAAACGGACTCGGTGTAGATCTCGG660.5248091603053435No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC580.4611959287531806No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA560.44529262086513993No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC510.40553435114503816No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT490.3896310432569975No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGG480.38167938931297707No Hit
GTCTATGATGCCCTTGTATTGCTTATCTGCCGTGATTTGCTTGCTGGCAT470.37372773536895676No Hit
CTCTTATACACATCTGACGCTGCCGACGAAACGGACTCGGTGTAGATCTC450.35782442748091603No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT440.34987277353689566No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGGACTCGGTG380.30216284987277353No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA320.2544529262086514No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG300.2385496183206107No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG300.2385496183206107No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGG290.2305979643765903No Hit
GTATATTATCTACCTTTTAAATGACATATAAACTCATTCACATCTATTAG280.22264631043256997No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC280.22264631043256997No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG270.21469465648854963No Hit
GTACAAGACCCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACT240.19083969465648853No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG230.1828880407124682No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC210.16698473282442747No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGGACTCGGTGTT200.1590330788804071No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT200.1590330788804071No Hit
GTGTGTACAAGACCCGGGAACGTATTCACCGCGGCATGCTGATCCGCGAT190.15108142493638677No Hit
GAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA190.15108142493638677No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC180.1431297709923664No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA180.1431297709923664No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACGGACTC180.1431297709923664No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAACG180.1431297709923664No Hit
GTGTACAAGACCCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTA170.13517811704834604No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC160.1272264631043257No Hit
CCTTTGTAGTGTCCGCGAACAACCTGAACTTCGTCATCCTTCCGAATGGG150.11927480916030535No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.11927480916030535No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA140.11132315521628498No Hit
CCCATGGTGTGACGGGCGGTGTGTACAAGACCCGGGAACGTATTCACCGC140.11132315521628498No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATAT140.11132315521628498No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA140.11132315521628498No Hit
CTCTTATACACATCTGACGCTGCCGACGAAACGGACTCGGTGTAGTTCTC140.11132315521628498No Hit
CAGCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCG140.11132315521628498No Hit
CTTATACACATCTGACGCTGCCGACGAAACGGACTCGGGGTAGATCTCGG130.10337150127226462No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCA130.10337150127226462No Hit
CATCTGACGCTGCCGACGAAACGGACTCGGTGTAGATCTCGGTGGTCGCC130.10337150127226462Illumina RNA PCR Primer (95% over 22bp)
ATACACATCTGACGCTGCCGACGAAACGGACTCGGTGTAGATCTCGGTGG130.10337150127226462No Hit
GAGTAAGGGTCCAAAACCAGTGCTGTGGTCCCTTTGTTGCCTGGGGGAGG130.10337150127226462No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)