FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007823019

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823019
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15783
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCACGGTACAATCG5613.554457327504277No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC3902.4710131153773047No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCACGGTACAATCT3472.198568079579294No Hit
ATTGCGCAATGGTTACCTGGGGGTTAGAAGCGAACCTGGGGAACTGAAAC2521.596654628397643No Hit
ATTGCGCAATGATTATGCGGGGAGCGAACCTGGGGAACTGAAACATCTAA2491.577646835202433No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2471.564974973072293No Hit
ATTGCGCAATGAAGCTATTGGGTAGAACACCTGTTGTCCTTGAGTAGGGC2051.2988658683393524No Hit
ATTGCGCAATGGATACGCCGGGGATAACAGGCTGATGACCCCCAAGAGTC1691.070772349996832No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1270.8046632452638915No Hit
ATTGCGCAATGGTAGATGTGGGGGGAAAGGATGTGAGGATCCCAAAACAA960.6082493822467212No Hit
ATTGCGCAATGTGATGTTCGGGGTGGGAAGATGAGAAGAGAGGGAAAAGA890.563897864791231No Hit
ATTGCGCAATGCGCAGACGGGGGTGGAAGAACCTGGAAAGGTTTGCCGAA890.563897864791231No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCACGGTAC890.563897864791231No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCACGGTACAAT800.506874485205601No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG760.4815307609453209No Hit
ATTGCGCAATGGGGAACGTGGGCAGCCATCATTTAAAGAAAGCGTAACAG660.4181714502946208No Hit
ATTGCGCAATGTGCGTAAGGGGCAGCCGCGGTAATACGAAGGGGGCTAGC650.41183551922955075No Hit
ATTGCGCAATGAAAACTATGGGAAGAAGTATGCTCTTGGTGCTGCTTGCA610.3864917949692708No Hit
ATTGCGCAATGTATTGAAGGGGGGTTAAGTTACTAAGGGCACACGGTGGA610.3864917949692708No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT580.3674840017740607No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC580.3674840017740607No Hit
ATTGCGCAATGTGAGCGGCGGGTTTATCGCGAGCTATTACCAGCAATACA570.3611480707089907No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT490.31046062218843057No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT490.31046062218843057No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGCAC460.29145282899322056No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG360.22809351834252042No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC360.22809351834252042No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT340.2154216562123804No Hit
ATTGCGCAATGTCGCTTACGGGGGTTCCAGGAAATAGCCCCTCCATTATA340.2154216562123804No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA300.19007793195210038No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACGCACGGTACAATCG280.17740606982196033No Hit
ATTGCGCAATGTTCCTCTCGGGGGCAATAGGGAATAAAGTGGAAGAACCT280.17740606982196033No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC270.1710701387568903No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG270.1710701387568903No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC260.16473420769182032No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC230.14572641449661028No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCACG230.14572641449661028No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG230.14572641449661028No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC220.13939048343154026No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG210.13305455236647026No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT210.13305455236647026No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG200.12671862130140024No Hit
ATTGCGCAATGAAACGGGGGGGGGTGGGGATGGAAATGTGTGGCATGCAC190.12038269023633022No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT190.12038269023633022No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCACGGTACAAGCG190.12038269023633022No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT190.12038269023633022No Hit
ATTGCGCAATGCTTGGACGGGGGATGCATCCTTATGGATGCGTGGTAGCG170.1077108281061902No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGCA170.1077108281061902No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT170.1077108281061902No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG170.1077108281061902No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACGCACGGTACAATCT160.1013748970411202No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT160.1013748970411202No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)