FastQCFastQC Report
Tue 7 May 2024
EGAF00007823020

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823020
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15783
Total Bases2.3 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCAGGTAGGGTGTA6063.839574225432427No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT5023.180637394665146No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC3021.9134511816511435No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCAGGTAGGGTGGA1420.8997022112399417No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCAGGTAGGGTGTT1350.8553506937844516No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCAGGTAGG900.5702337958563011No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCAGGTAGGGTG740.4688588988151809No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT600.38015586390420075No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCAGGTAGGGTGGT570.3611480707089907No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530.33580434644871066No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG460.29145282899322056No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC460.29145282899322056No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC430.2724450357980105No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA400.2534372426028005No Hit
GCTTTTCACCTTTCCCTCACGGTACTGGTTCGCTATCGGTCATGCACGAG380.24076538047266044No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG360.22809351834252042No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCAG360.22809351834252042No Hit
GTACTGGTTCGCTATCGGTCATGCACGAGTACTTAGGCTTGGATCGTGGT320.2027497940822404No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG310.19641386301717037No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC270.1710701387568903No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA260.16473420769182032No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT260.16473420769182032No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG260.16473420769182032No Hit
CTTATACACATCTGACGCTGCCGACGATTCAGGTAGGGTGTAGATCTCGG260.16473420769182032No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC250.1583982766267503No Hit
GGCTTGGATCGTGGTCGACCCATGTTCAGACAGGATTTCACGTGTCCCGC240.1520623455616803No Hit
CCTCCAGGATGTCTCACGACTGTCCCTTCACAGGCTTACGGAACGCTCCG240.1520623455616803No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT240.1520623455616803No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC240.1520623455616803No Hit
GTTCAGACTCGCTTTCGCTGCGCCTACACCTACCGGCTTAAGCTTGCTCG230.14572641449661028No Hit
TCTCACGACTGTCCCTTCACAGGCTTACGGAACGCTCCGCTACCACGCAC230.14572641449661028No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA220.13939048343154026No Hit
GTTTCACTCCCCTCGTCGGGGTGCTTTTCACCTTTCCCTCACGGTACTGG220.13939048343154026No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA180.11404675917126021No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC180.11404675917126021No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA170.1077108281061902No Hit
GTCATGCACGAGTACTTAGGCTTGGATCGTGGTCGACCCATGTTCAGACA160.1013748970411202No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGA160.1013748970411202No Hit
GGATTTCACGTGTCCCGCCCTACTCAAGGACAACAGGTGTTCTACGTGTA160.1013748970411202No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATA160.1013748970411202No Hit
CTTATACACATCTGACGCTGCCGACGATTCAGGTAGGGTGTAGTTCTCGG160.1013748970411202No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA160.1013748970411202No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.1013748970411202No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)