FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007823021

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823021
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11321
Sequences flagged as poor quality0
Sequence length151
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCATCGGCATCG5735.061390336542708No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCATCGGCATCT3823.374260224361805No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC3733.2947619468244858No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2121.8726260931013161No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA840.7419839236816536No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCATCGG810.7154844978358802No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG790.6978182139386979No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACAACCATCGGCATCG790.6978182139386979No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCATCGGCAT760.6713187880929247No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC760.6713187880929247No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT590.5211553749668757No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAACC570.5034890910696934No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT510.4504902393781468No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT470.41515767158378236No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT400.35332567794364456No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG360.3179931101492801No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCA350.309159968200689No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG340.3003268262520979No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG340.3003268262520979No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG340.3003268262520979No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC320.28266054235491567No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA310.27382740040632453No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC300.26499425845773344No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACAACCATCGGCATCT270.23849483261196008No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC250.22082854871477786No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT250.22082854871477786No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC240.21199540676618672No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCATCGGCAGCG240.21199540676618672No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC230.20316226481759564No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG230.20316226481759564No Hit
ATTGCGCAATGTCGTAGCGGGGTGGAAAAAAAATACAACCCAAAAAAAAA190.16782969702323117No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT190.16782969702323117No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT180.15899655507464006No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCATCGGCATCGCG170.15016341312604894No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG170.15016341312604894No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC160.14133027117745783No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT160.14133027117745783No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCATCG160.14133027117745783No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCCT160.14133027117745783No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT150.13249712922886672No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAC150.13249712922886672No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC150.13249712922886672No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTATGCCTGT140.1236639872802756No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG140.1236639872802756No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTGA130.11483084533168447No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA130.11483084533168447No Hit
ATTGCGCAATGGAAAGGCGGGGCAAGAGCAAAGAAAAATCCCTTTGGTTC130.11483084533168447No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGCT130.11483084533168447No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTG120.10599770338309336No Hit
ATTGCGCAATGTGGCCAGAGGGGAAAGAATATGCGCGCACGTCAAGAAAT120.10599770338309336No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACC120.10599770338309336No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)