FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007823022

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823022
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11321
Sequences flagged as poor quality0
Sequence length151
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT4544.010246444660366No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAACGAATAGTGTA3413.0121014044695698No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAACGAATAGTGTT2782.45561346170833No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAACGAATAGTGGT2602.2966169066336897No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC2572.2701174807879165No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAACGAATAGTGGA1731.5281335571062629No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAACGAATA790.6978182139386979No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAACGAATAGTG740.6536525041957424No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC500.4416570974295557No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG490.43282395548096453No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAAC470.41515767158378236No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT470.41515767158378236No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC430.3798251037894179No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA370.3268262520978712No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350.309159968200689No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG310.27382740040632453No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT300.26499425845773344No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC300.26499425845773344No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG290.2561611165091423No Hit
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAACG280.2473279745605512No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAACGAATAGTGGG260.22966169066336894No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC240.21199540676618672No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC240.21199540676618672No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240.21199540676618672No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240.21199540676618672No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG230.20316226481759564No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAACGAAT200.17666283897182228No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAC190.16782969702323117No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAACGAATAGTGTG180.15899655507464006No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA160.14133027117745783No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA160.14133027117745783No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC160.14133027117745783No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA150.13249712922886672No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATAT140.1236639872802756No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAA140.1236639872802756No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG140.1236639872802756No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAA130.11483084533168447No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)