FastQCFastQC Report
Tue 7 May 2024
EGAF00007823026

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823026
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7760
Total Bases1.1 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT3995.141752577319587No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTA3214.136597938144329No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC2062.654639175257732No Hit
CTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGG781.0051546391752577No Hit
CTCTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTC660.8505154639175257No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT610.7860824742268041No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC520.6701030927835051No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT480.6185567010309279No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAG400.5154639175257731No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGTCTCCTAGG370.47680412371134023No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGTCTC310.3994845360824742No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG280.36082474226804123No Hit
ATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGTGG260.33505154639175255No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC240.30927835051546393No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA240.30927835051546393No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGTGGTCGCC230.2963917525773196Illumina Single End PCR Primer 1 (100% over 21bp)
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC230.2963917525773196No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTG220.28350515463917525No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG200.25773195876288657No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.24484536082474226No Hit
CTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGTTCTCGG180.23195876288659795No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGTCTC160.2061855670103093No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG160.2061855670103093No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA160.2061855670103093No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG150.19329896907216496No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC130.16752577319587628No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGTGGTCGGC130.16752577319587628Illumina Single End PCR Primer 1 (95% over 21bp)
CTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTATATCTCGG130.16752577319587628No Hit
GTCCGTATACATCGTCTTACGACTTCGCACGGACCTGTGTTTTTGGTAAA120.15463917525773196No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC120.15463917525773196No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC120.15463917525773196No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA120.15463917525773196No Hit
ATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGGGG110.14175257731958762No Hit
GTCTCTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATC110.14175257731958762No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGTGGGCGCC110.14175257731958762Illumina Single End PCR Primer 1 (95% over 21bp)
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGTGGTGGCC110.14175257731958762Illumina Single End PCR Primer 1 (95% over 21bp)
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGTGGTGGGC110.14175257731958762No Hit
CTCTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGTTCTC100.12886597938144329No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATAT100.12886597938144329No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT90.11597938144329897No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGTCTCCTAG90.11597938144329897No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATATGTA90.11597938144329897No Hit
GTTCGCTACCCATGCTTTCGCTCCTCAGCGTCAGTAACTGCCCAGTAACC90.11597938144329897No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.11597938144329897No Hit
CCCCTGGACTCTGCGCCCCTCACCGCCACCACACCGCACGGGTGCACGAC90.11597938144329897No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.11597938144329897No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAA80.10309278350515465No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG80.10309278350515465No Hit
GGATATAGCTGTACTTGCGGCTGACTGAGCGCTGCCGGATTGTGTGCTCG80.10309278350515465No Hit
CAGCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCG80.10309278350515465No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)