FastQCFastQC Report
Tue 7 May 2024
EGAF00007823030

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823030
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6584
Total Bases994.1 kbp
Sequences flagged as poor quality0
Sequence length151
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT3425.194410692588092No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTA2754.1767922235722965No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1832.779465370595383No Hit
CTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGG981.488456865127582No Hit
CTCTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTC891.3517618469015795No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAG420.637910085054678No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGTCTCCTAGG390.5923450789793439No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT380.5771567436208992No Hit
ATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGTGG310.47083839611178613No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.44046172539489675No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC290.44046172539489675No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGTGGTCGCC270.4100850546780073Illumina Single End PCR Primer 1 (100% over 21bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC230.3493317132442284No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTG230.3493317132442284No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG220.33414337788578374No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG220.33414337788578374No Hit
CTCTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTATATCTC200.3037667071688943No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGTCTC200.3037667071688943No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA180.2733900364520049No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC180.2733900364520049No Hit
CTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGTTCTCGG160.24301336573511542No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGTGGTGGCC150.2278250303766707Illumina Single End PCR Primer 1 (95% over 21bp)
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.2278250303766707No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG140.212636695018226No Hit
CTCTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGTTCTC130.19744835965978128No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA120.18226002430133656No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.18226002430133656No Hit
CTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCGCGG110.16707168894289187No Hit
ATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGGGG110.16707168894289187No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC110.16707168894289187No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG110.16707168894289187No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGTCTC100.15188335358444716No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG100.15188335358444716No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGTGGGGGCC100.15188335358444716No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA100.15188335358444716No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGGGGTCGCC90.13669501822600244Illumina Single End PCR Primer 1 (95% over 21bp)
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGTGGTGGGC90.13669501822600244No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA80.12150668286755771No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATATTT80.12150668286755771No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAA80.12150668286755771No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC80.12150668286755771No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA80.12150668286755771No Hit
CACATACGGTTTAGCCCCATCCGCGTTCGCTCGCCACTACTAACGGAATC70.106318347509113No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT70.106318347509113No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGA70.106318347509113No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC70.106318347509113No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA70.106318347509113No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGGGGGCGCC70.106318347509113No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCA70.106318347509113No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAA70.106318347509113No Hit
GTCTCTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATC70.106318347509113No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGTCT70.106318347509113No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATAT70.106318347509113No Hit
GCATACGCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACG70.106318347509113No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.106318347509113No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAAT70.106318347509113No Hit
CTCTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATATC70.106318347509113No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)