FastQCFastQC Report
Tue 7 May 2024
EGAF00007823034

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823034
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5477
Total Bases827 kbp
Sequences flagged as poor quality0
Sequence length151
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTA2063.7611831294504285No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT1793.2682125251049845No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1272.318787657476721No Hit
CTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGG761.38762096037977No Hit
CTCTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTC591.077232061347453No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGTCTCCTAGG540.9859412086908891No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT480.8763921855030126No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT470.8581340149716999No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGTGGTCGCC330.6025196275333212Illumina Single End PCR Primer 1 (100% over 21bp)
ATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGTGG280.5112287748767573No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC280.5112287748767573No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGTCTC260.4747124338141318No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTG230.41993792222019355No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG230.41993792222019355No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAG180.3286470695636297No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG170.310388899032317No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG170.310388899032317No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG170.310388899032317No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC160.29213072850100424No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC160.29213072850100424No Hit
CTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGTTCTCGG160.29213072850100424No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC150.2738725579696914No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.25561438743837867No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGTCT120.21909804637575314No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.21909804637575314No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCA100.18258170531312762No Hit
ATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGGGG100.18258170531312762No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC100.18258170531312762No Hit
CTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCGCGG90.16432353478181486No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTATATCTCGGTGGTCGCC90.16432353478181486Illumina Single End PCR Primer 1 (95% over 21bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC90.16432353478181486No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA90.16432353478181486No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC90.16432353478181486No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGTGGTGGCC90.16432353478181486Illumina Single End PCR Primer 1 (95% over 21bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA90.16432353478181486No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGTCTC80.14606536425050212No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAAATGT80.14606536425050212No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAAATTT80.14606536425050212No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGGGGGCGCC80.14606536425050212No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT80.14606536425050212No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA80.14606536425050212No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGGGGTCGCC80.14606536425050212Illumina Single End PCR Primer 1 (95% over 21bp)
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGTGGGCGCC80.14606536425050212Illumina Single End PCR Primer 1 (95% over 21bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA80.14606536425050212No Hit
ATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGTTCTCGGTGG80.14606536425050212No Hit
CTCTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTATATCTC80.14606536425050212No Hit
CTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTATATCTCGG80.14606536425050212No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA80.14606536425050212No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA80.14606536425050212No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGTGGGGGCC70.12780719371918933No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGTGGTCGGC70.12780719371918933Illumina Single End PCR Primer 1 (95% over 21bp)
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA70.12780719371918933No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGTGGTGGGC70.12780719371918933No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC70.12780719371918933No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGTCTCCTAG60.10954902318787657No Hit
TCTGTCTCTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAG60.10954902318787657No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAAAATGT60.10954902318787657No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAAAATTT60.10954902318787657No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGTGGGGGGC60.10954902318787657No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA60.10954902318787657No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGTTCTCGGTGGTGGCC60.10954902318787657No Hit
CTCTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGTTCTC60.10954902318787657No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGGGGGGGGC60.10954902318787657No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA60.10954902318787657No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.10954902318787657No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)