FastQCFastQC Report
Mon 16 Jan 2023
EGAF00007823039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5565
Sequences flagged as poor quality0
Sequence length151
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC3356.019766397124888No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1773.1805929919137466No Hit
ATTGCGCAATGAGTGACAAGGGGGAGAGGCTTTTCTCGTTGGCAACCAGC851.527403414195867No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA370.6648697214734951No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG340.6109613656783468No Hit
ATTGCGCAATGGGGTTAATGGGCACGTAGGCGGATTGTTAAGTGAGGGGT330.5929919137466307No Hit
ATTGCGCAATGGGAGAAGGGGGAGACTTCAAGCCACAAGGAAAGAAGACG290.5211141060197664No Hit
ATTGCGCAATGAGGATGGGGGGCAAGCTGCGGAGGGACGCAGTGAGGTAG280.5031446540880503No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC250.44923629829290207No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGATGGAGA180.3234501347708895No Hit
ATTGCGCAATGCGAGGGTGGGGGAGACTTCAAGCCACAAGGAAAGAAGAC180.3234501347708895No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGATGGAGAAATCG140.25157232704402516No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTGAT120.215633423180593No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA110.1976639712488769No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA100.17969451931716085No Hit
ATTGCGCAATGAAGGGCAGGGGCGTGGCGCTGCCCGGGGTGGGAGTGAGC90.16172506738544476No Hit
ATTGCGCAAGCAGAAGACGGCATACGAGATTCTGACTGTGACTGGAGTTC90.16172506738544476RNA PCR Primer, Index 40 (96% over 29bp)
ATTGCGCAATCTGTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGA90.16172506738544476No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGATGGAGAAAT80.14375561545372867No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT70.12578616352201258No Hit
ATTGCGCAATGCACATACGGGGGAACTCGGCAAATTGCCTCCGTACCTTC60.1078167115902965No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGATGGAGAAATCT60.1078167115902965No Hit
ATTGCGCAATGGACGGAGGGGGAGGATTACCAATCTCAGATAAACTCCGA60.1078167115902965No Hit
GGGGTGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60.1078167115902965No Hit
ATTGCGCAATGTATGCTGCTGCTGTCTCTTATACACATCTCCGAGCCCAC60.1078167115902965No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)