FastQCFastQC Report
Mon 16 Jan 2023
EGAF00007823040

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823040
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5565
Sequences flagged as poor quality0
Sequence length151
%GC55

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT4417.9245283018867925No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC2504.492362982929021No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTA1652.964959568733154No Hit
CTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGG771.3836477987421385No Hit
CTCTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTC510.9164420485175202No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC480.862533692722372No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT450.8086253369272237No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT390.7008086253369272No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGTCTCCTAGG360.646900269541779No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAG360.646900269541779No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGTGGTCGCC250.44923629829290207Illumina Single End PCR Primer 1 (100% over 21bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC240.431266846361186No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGTCTC230.4132973944294699No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTG210.37735849056603776No Hit
ATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGTGG190.3414195867026056No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG180.3234501347708895No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC180.3234501347708895No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.3054806828391734No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA160.28751123090745734No Hit
GTTTAGGATCTTACTGTCTTGGAATGTCCTTAGAAGGCTCTGTCATTGAA150.2695417789757413No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG150.2695417789757413No Hit
CTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGTTCTCGG150.2695417789757413No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG140.25157232704402516No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGTGGGGGCC130.23360287511230907No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA110.1976639712488769No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.1976639712488769No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC100.17969451931716085No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGTGGTGGCC100.17969451931716085Illumina Single End PCR Primer 1 (95% over 21bp)
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGTGGTGGGC100.17969451931716085No Hit
GAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA90.16172506738544476No Hit
CTCTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGTTCTC90.16172506738544476No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA90.16172506738544476No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGTGGGCGCC90.16172506738544476Illumina Single End PCR Primer 1 (95% over 21bp)
CTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATATCGG90.16172506738544476No Hit
GTCTTGGAATGTCCTTAGAAGGCTCTGTCATTGAACAGGTAACAGAGTAA80.14375561545372867No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG80.14375561545372867No Hit
CTATATACCTTGCTTCTGGAATGCTCTGCAGCTGGCCTGTGGTGACACTG80.14375561545372867No Hit
GTCCTGACCACCTCAACATAGGGACCATCTGGAGGAGGCTTCCTCTGACT80.14375561545372867No Hit
CCTTAAAGCACGCTGCACTAGAACTTCAGGACAGTCCTGACCACCTCAAC80.14375561545372867No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.14375561545372867No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA80.14375561545372867No Hit
CAGCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCG80.14375561545372867No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA70.12578616352201258No Hit
ATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGGGG70.12578616352201258No Hit
CCTTAGAAGGCTCTGTCATTGAACAGGTAACAGAGTAAAAACCCCAGAGC70.12578616352201258No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGGGGTCGCC70.12578616352201258Illumina Single End PCR Primer 1 (95% over 21bp)
GCATACGCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACG70.12578616352201258No Hit
CTCTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTATATCTC70.12578616352201258No Hit
CTCTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATATC70.12578616352201258No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA60.1078167115902965No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATATGT60.1078167115902965No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC60.1078167115902965No Hit
CATCAGCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTG60.1078167115902965No Hit
ACATAGGGACCATCTGGAGGAGGCTTCCTCTGACTTCCGGGTTGCAGGAA60.1078167115902965No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGTGGTCGGC60.1078167115902965Illumina Single End PCR Primer 1 (95% over 21bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCA60.1078167115902965No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAA60.1078167115902965No Hit
GTACAGGGGCACTGAGTTCTTCCACCATCAAAATGGCTTCTAGGAGCTGG60.1078167115902965No Hit
CTCTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTA60.1078167115902965No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGTCT60.1078167115902965No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC60.1078167115902965No Hit
CCTCAACATAGGGACCATCTGGAGGAGGCTTCCTCTGACTTCCGGGTTGC60.1078167115902965No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)