FastQCFastQC Report
Tue 17 Jan 2023
EGAF00007823042

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823042
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9712
Sequences flagged as poor quality0
Sequence length151
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTA5705.869028006589786No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT2752.8315485996705108No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1501.544481054365733No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1131.163509060955519No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGTCTCCTAGG870.8957990115321252No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT860.8855024711696869No Hit
CTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGG600.6177924217462932No Hit
CTCTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTC600.6177924217462932No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAG440.4530477759472817No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTG400.41186161449752884No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGTCTC370.38097199341021415No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC330.3397858319604613No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG300.3088962108731466No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG270.278006589785832No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270.278006589785832No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA250.2574135090609555No Hit
GGTATATATGTACAAGGACTGGATGGCATGGCCAGGCAAATCAGATGGAA240.2471169686985173No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC240.2471169686985173No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA230.23682042833607905No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.22652388797364084No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA200.20593080724876442No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGTGGTCGCC200.20593080724876442Illumina Single End PCR Primer 1 (100% over 21bp)
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC200.20593080724876442No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG190.19563426688632618No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC190.19563426688632618No Hit
ATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGTGG170.17504118616144976No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGTCTC170.17504118616144976No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGTCT160.16474464579901155No Hit
CTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGTTCTCGG160.16474464579901155No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT150.1544481054365733No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.1544481054365733No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGTCTCCTAG140.1441515650741351No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG140.1441515650741351No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC130.13385502471169688No Hit
CCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTCATTGAAT130.13385502471169688No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCA120.12355848434925865No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGTGGGCGCC120.12355848434925865Illumina Single End PCR Primer 1 (95% over 21bp)
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA120.12355848434925865No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGA110.11326194398682042No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA110.11326194398682042No Hit
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGTCTCC110.11326194398682042No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCC100.10296540362438221No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAAT100.10296540362438221No Hit
CCCATGGACTGTCTCCCGTAATTGAACTTCTCAGTATTCGGAGTTTGAAA100.10296540362438221No Hit
CTCTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGTTCTC100.10296540362438221No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGGGGTCGCC100.10296540362438221Illumina Single End PCR Primer 1 (95% over 21bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC100.10296540362438221No Hit
CAGCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCG100.10296540362438221No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)