FastQCFastQC Report
Tue 7 May 2024
EGAF00007823049

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823049
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4531
Total Bases684.1 kbp
Sequences flagged as poor quality0
Sequence length151
%GC60

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC2906.40035312293092No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1022.2511586846170824No Hit
ATTGCGCAATGTAGTCACGGGGAAGGCTGCTGTCGTGAACATGATCAAGG661.4566320900463474No Hit
ATTGCGCAATGCGCCTACAGGGCGGTGTGAGTAGCGAAAGACGGGTGAGA491.0814389759435004No Hit
ATTGCGCAATGATGTATCTGGGGATAATATGAAAGATATATTTCGTGTGT471.0372986095784595No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG430.9490178768483779No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.5517545795630104No Hit
ATTGCGCAATGAGGTCGTGGGGCGGCAAATTGCCTCCGTACCTTCGGAAG150.33105274773780624No Hit
GGGGTGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG110.24277201500772458No Hit
ATTGCGCAATCTGTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGA80.17656146546016332No Hit
GGGGGCTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG70.15449128227764292No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC70.15449128227764292No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCAGCTATATCG70.15449128227764292No Hit
ATTGCGCAATATGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC70.15449128227764292No Hit
GGGGGGTGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60.13242109909512248No Hit
ATTGCGCAATGATCTCAGTGGGAAGGATAAAAAAAAATAAAAGAAAAAAT60.13242109909512248No Hit
ATTGCGCAAGCAGAAGACGGCATACGAGATTCTGACTGTGACTGGAGTTC60.13242109909512248RNA PCR Primer, Index 40 (96% over 29bp)
GCGTGCGGGCTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60.13242109909512248No Hit
GGGGTGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50.11035091591260207No Hit
ATTGCGCAATCTGTCTCTTATACACCTGGCTCTTATACACATCTCCGAGC50.11035091591260207No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)