FastQCFastQC Report
Mon 16 Jan 2023
EGAF00007823050

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823050
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4531
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT3808.386669609357758No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1753.8622820569410727No Hit
CTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGG962.1187375855219597No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTA591.3021408077687044No Hit
CTCTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTC541.1917898918561023No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC541.1917898918561023No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT390.8607371441182963No Hit
ATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGTGG340.7503862282056941No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280.6179651291105717No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGTGGTCGCC250.5517545795630104Illumina Single End PCR Primer 1 (100% over 21bp)
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGTGGGGGCC170.37519311410284706No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGTCTCCTAGG160.35312293092032665No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGTGGTGGCC150.33105274773780624Illumina Single End PCR Primer 1 (95% over 21bp)
CTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGTTCTCGG150.33105274773780624No Hit
GTACAGTAGATACCGTCAAGGAACTTGCGGATATCCTTGTTGCGCACGAG140.30898256455528583No Hit
CCTGTATACAGCGTATCATGCGCCCTCTACACAGGGTATTTTTTAAGCGT120.26484219819024496No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGGGGTCGCC120.26484219819024496Illumina Single End PCR Primer 1 (95% over 21bp)
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGTGGGCGCC120.26484219819024496Illumina Single End PCR Primer 1 (95% over 21bp)
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.26484219819024496No Hit
CTCTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGTTCTC110.24277201500772458No Hit
ATCCTACTGCGTCCCCCCATCGATTAAAACGATTATAGGTGGTACAGGAA110.24277201500772458No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGTGGGGGGC110.24277201500772458No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGTGGTCGGC110.24277201500772458Illumina Single End PCR Primer 1 (95% over 21bp)
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.24277201500772458No Hit
CTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCGCGG100.22070183182520414No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG100.22070183182520414No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG100.22070183182520414No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC100.22070183182520414No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC100.22070183182520414No Hit
GTCTCTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATC90.19863164864268373No Hit
ATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGTTCTCGGTGG90.19863164864268373No Hit
CTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATATCGG90.19863164864268373No Hit
CTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTATATCTCGG90.19863164864268373No Hit
ATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGGGG80.17656146546016332No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG80.17656146546016332No Hit
CTCTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTATATCTC80.17656146546016332No Hit
CTGCGAGACCTCGTCAATGTCATTGCCCTCAACGAGAACCTGGTCCTTCT80.17656146546016332No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAG70.15449128227764292No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG70.15449128227764292No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGGGGTGGCC70.15449128227764292No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA70.15449128227764292No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGGGGGCGCC70.15449128227764292No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.15449128227764292No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC70.15449128227764292No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC70.15449128227764292No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGTCTC70.15449128227764292No Hit
ATCTTAAGAGTGTCGGATTTGCCTAACACTCAATCTCACTGCTTAGACGT60.13242109909512248No Hit
GAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA60.13242109909512248No Hit
ATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.13242109909512248No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA60.13242109909512248No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGGGGTGGGC60.13242109909512248No Hit
GCCTAACACTCAATCTCACTGCTTAGACGTACAATCCAATCGTACGCTTC60.13242109909512248No Hit
GCTTAGACGTACAATCCAATCGTACGCTTCGCCTATCCTACTGCGTCCCC60.13242109909512248No Hit
GCGTCACACCGTGAAGCATGGCAGCGCTCTGCGAGACCTCGTCAATGTCA60.13242109909512248No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGTTCTCGGTGGTCGCC50.11035091591260207Illumina Single End PCR Primer 1 (95% over 21bp)
CCCCATCACAGCTCAATCTTAAGAGTGTCGGATTTGCCTAACACTCAATC50.11035091591260207No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGTTCTCGGGGGTCGCC50.11035091591260207No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAAATGT50.11035091591260207No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC50.11035091591260207No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG50.11035091591260207No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTG50.11035091591260207No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGGGGTCGGC50.11035091591260207No Hit
CATCTGACGCTGCCGACGAGTCTCCTAGGGTGTAGATCTCGGGGGGCGGC50.11035091591260207No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCA50.11035091591260207No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)