FastQCFastQC Report
Tue 7 May 2024
EGAF00007823053

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823053
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3502
Total Bases528.8 kbp
Sequences flagged as poor quality0
Sequence length151
%GC63

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC2336.653340948029697No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC992.826956025128498No Hit
ATTGCGCAATGAGTTATAAGGGGACTCCTGTCCCAGCCACCGTCAGCCAC260.7424328954882925No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.6567675613934895No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG210.5996573386636208No Hit
ATTGCGCAATGGATCGACTGGGGGAGAGTTTGATTCTGGCTCAGGACGAA170.48543689320388345No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC110.31410622501427754No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCGTGCTGATCT60.17133066818960593No Hit
GGGGGGTGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60.17133066818960593No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTC50.1427755568246716No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCC50.1427755568246716No Hit
ATTGCGCAATGTTCGATCCGGGTCATAGAGGGTGAGAATCCCGTCTGGCG50.1427755568246716No Hit
GGGGTGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50.1427755568246716No Hit
GGGGGCTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50.1427755568246716No Hit
ATTGCGCAATCTGTCTCTTATACACCTGGCTCTTATACACATCTCCGAGC50.1427755568246716No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCC50.1427755568246716No Hit
GGGGGCTGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50.1427755568246716No Hit
ATTGCGCAATGGTACAACGGGGCATCTAAAACTTAGCTTAAAATTTACAG50.1427755568246716No Hit
ATTGCGCAAGCAGAAGACGGCATACGAGATTCTGACTGTGACTGGAGTTC40.1142204454597373RNA PCR Primer, Index 40 (96% over 29bp)
ATTGCGCAATCTGTCTCTTATCTGTCTCTTATACACATCTCCGAGCCCAC40.1142204454597373No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC40.1142204454597373No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT40.1142204454597373No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCGAGCC40.1142204454597373No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCGCTTATACACATCTCCGAGC40.1142204454597373No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCGTGCT40.1142204454597373No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)