FastQCFastQC Report
Thu 19 Jan 2023
EGAF00007823056

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823056
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128466
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTAGGATGATGGCGGGCAGGATAGTTCAGACGGTTTCTATTTCCTGA7130.5550106643002818No Hit
GTACCATTGGTGGCCAATTGATTTGATGGTAAGGGAGGGATCGTTGACCT5660.4405835006927903No Hit
GTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTCGTAGGTT5530.43046409166627747No Hit
GTTCAGTACCATTGGTGGCCAATTGATTTGATGGTAAGGGAGGGATCGTT4770.3713044696651254No Hit
GGATAGTTCAGACGGTTTCTATTTCCTGAGCGTCTGAGATGTTAGTATTA4730.3681908053492753No Hit
GGAGTAGGAGAGAGGGAGGTAAGAGTCAGAAGCTTATGTTGTTTATGCGG4660.36274189279653757No Hit
CTCGTAGGTTCAGTACCATTGGTGGCCAATTGATTTGATGGTAAGGGAGG3960.30825276726916073No Hit
GAGTAGGAGAGAGGGAGGTAAGAGTCAGAAGCTTATGTTGTTTATGCGGG3880.3020254386374605No Hit
GTGTACTCGTAGGTTCAGTACCATTGGTGGCCAATTGATTTGATGGTAAG3640.28334345274235984No Hit
GTTATGTAAAGGATGCGTAGGGATGGGAGGGCGATGAGGACTAGGATGAT3540.27555929195273454No Hit
ATGTAAAGGATGCGTAGGGATGGGAGGGCGATGAGGACTAGGATGATGGC3490.2716672115579219No Hit
GTATTACTATGAAGAAGATTATTACAAATGCATGGGCTGTGACGATAACG3260.25376364174178384No Hit
GCTCATGAGTGCAAGACGTCTTGTGATGTAATTATTATACGAATGGGGGC2910.22651907897809537No Hit
GTTCTAGGAATAATGGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCG2910.22651907897809537No Hit
GTTCAGACGGTTTCTATTTCCTGAGCGTCTGAGATGTTAGTATTAGTTAG2860.22262699858328272No Hit
GACTAGGATGATGGCGGGCAGGATAGTTCAGACGGTTTCTATTTCCTGAG2600.20238818053025703No Hit
CGTCTGTTATGTAAAGGATGCGTAGGGATGGGAGGGCGATGAGGACTAGG2590.2016097644512945No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2560.19927451621440692No Hit
GGATGATGGCGGGCAGGATAGTTCAGACGGTTTCTATTTCCTGAGCGTCT2460.19149035542478166No Hit
GCGTAGGGATGGGAGGGCGATGAGGACTAGGATGATGGCGGGCAGGATAG2410.187598275029969No Hit
ATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTCGTAGGTTCA2340.18214936247723132No Hit
GCCGTAGTCGGTGTACTCGTAGGTTCAGTACCATTGGTGGCCAATTGATT2310.17981411424034374No Hit
CCACTATAGCAGATGCGAGCAGGAGTAGGAGAGAGGGAGGTAAGAGTCAG2300.17903569816138123No Hit
CTATAGCAGATGCGAGCAGGAGTAGGAGAGAGGGAGGTAAGAGTCAGAAG2300.17903569816138123No Hit
GTGTAGGAAGAGGCAGATAAAGAATATTGAGGCGCCATTGGCGTGAAGGT2280.17747886600345616No Hit
TCTGAGATGTTAGTATTAGTTAGTTTTGTTGTGAGTGTTAGGAAAAGGGC2270.17670044992449363No Hit
GTAAGGGAGGGATCGTTGACCTCGTCTGTTATGTAAAGGATGCGTAGGGA2250.1751436177665686No Hit
GTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGG2040.15879688010835552No Hit
TGTGGGGACAGCTCATGAGTGCAAGACGTCTTGTGATGTAATTATTATAC2040.15879688010835552No Hit
GGGCAGGATAGTTCAGACGGTTTCTATTTCCTGAGCGTCTGAGATGTTAG2030.15801846402939299No Hit
GGGAGGGATCGTTGACCTCGTCTGTTATGTAAAGGATGCGTAGGGATGGG1980.15412638363458037No Hit
GGGATGGGAGGGCGATGAGGACTAGGATGATGGCGGGCAGGATAGTTCAG1890.1471206389239176No Hit
GTTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAAT1890.1471206389239176No Hit
CGTAGGTTCAGTACCATTGGTGGCCAATTGATTTGATGGTAAGGGAGGGA1850.1440069746080675No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG1840.14322855852910496No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGTCTCCTAGGGTGTA1840.14322855852910496No Hit
GGACTAGGAAGCAGATAAGGAAAATGATTATGAGGGCGTGATCATGAAAG1840.14322855852910496No Hit
ACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGTTTGCTAATAC1820.14167172637117992No Hit
GGGTATTACTATGAAGAAGATTATTACAAATGCATGGGCTGTGACGATAA1770.13777964597636727No Hit
GTGTTAGGAAAAGGGCATACAGGACTAGGAAGCAGATAAGGAAAATGATT1700.13233073342362958No Hit
AGGGTAGACTGTTCAACCTGTTCCTGCTCCGGCCTCCACTATAGCAGATG1690.13155231734466707No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT1680.13077390126570454No Hit
ATATAGGCCTCGCCCGATGTGTAGGAAGAGGCAGATAAAGAATATTGAGG1650.12843865302881696No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGAGTGATAT1640.12766023694985443No Hit
TTGTAGATGTGGTCGTTACCTAGAAGGTTGCCTGGCTGGCCCAGCTCGGC1630.12688182087089192No Hit
GTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTCGCCTGGTTCTAGG1600.12454657263400433No Hit
GTACAAGGAAGGGGTAGGCTATGTGTTTTGTCAGGGGGTTGAGAATGAGT1600.12454657263400433No Hit
GAATAATGGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGG1540.11987607616022918No Hit
GTGTAGTACGATGTCTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGC1530.11909766008126664No Hit
GTCTACTGAGTAGCCTCCTCAGATTCATTGAACTAGGTCTGTCCCAATGT1500.11676241184437905No Hit
GATTATTACAAATGCATGGGCTGTGACGATAACGTTGTAGATGTGGTCGT1470.11442716360749149No Hit
GTATTAGTTAGTTTTGTTGTGAGTGTTAGGAAAAGGGCATACAGGACTAG1440.1120919153706039No Hit
GTCTAGGCCATATGTGTTGGAGATTGAGACTAGTAGGGCTAGGCCCACCG1430.11131349929164136No Hit
GTTCCTGCTCCGGCCTCCACTATAGCAGATGCGAGCAGGAGTAGGAGAGA1410.1097566671337163No Hit
TGCATGGGCTGTGACGATAACGTTGTAGATGTGGTCGTTACCTAGAAGGT1410.1097566671337163No Hit
GTTTTGTTGTGAGTGTTAGGAAAAGGGCATACAGGACTAGGAAGCAGATA1400.1089782510547538No Hit
CTACTCGATTGTCAACGTCAAGGAGTCGCAGGTCGCCTGGTTCTAGGAAT1390.10819983497579125No Hit
TCGTTGACCTCGTCTGTTATGTAAAGGATGCGTAGGGATGGGAGGGCGAT1380.10742141889682874No Hit
TGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAAAGAAAGATGAATC1350.10508617065994115No Hit
GGGTAGAGGGGGTGTTATAGGGTAAATACGGGCCCTATTTCAAAGATTTT1330.1035293385020161No Hit
GTATTATACCATAGCCGCCTAGTTTTAAGAGTACTGCGGCAAGTACTATT1320.10275092242305356No Hit
GTCTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGCCACCTACGGTGA1320.10275092242305356No Hit
GCTAGGAATAGTCCTGTGGTGATTTGGAGGATCAGGCAGGCGCCAAGGAG1310.10197250634409105No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)