FastQCFastQC Report
Mon 16 Jan 2023
EGAF00007823057

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823057
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4110
Sequences flagged as poor quality0
Sequence length151
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC1904.622871046228711No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1293.1386861313868613No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA421.0218978102189782No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG280.6812652068126521No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC190.4622871046228711No Hit
ATTGCGCAATGAGCTCTTGGGGGAACTTGGAGATAGCTGGTTCTCTCCGA180.43795620437956206No Hit
AGGCTACACTGATTAAGAAATGGGATCACTCAGGAGGATTGCTTGAGCCC160.38929440389294406No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGATCTCT140.34063260340632606No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGA130.31630170316301703No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTAGATCTCTCATCG120.291970802919708No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGATCTCTCATCG90.21897810218978103No Hit
ATTGCGCAATGTGACGATGGGGGCGAAAGAAAGAGGAAAGGGAAGAAAAA90.21897810218978103No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA90.21897810218978103No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGATCTCTCATCT80.19464720194647203No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTAGGTCTCTCATCG80.19464720194647203No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC80.19464720194647203No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG80.19464720194647203No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA70.17031630170316303No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT70.17031630170316303No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG60.145985401459854No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT50.12165450121654502No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGATCTCTCAT50.12165450121654502No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA50.12165450121654502No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)