FastQCFastQC Report
Tue 7 May 2024
EGAF00007823064

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823064
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10815
Total Bases1.6 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT4874.503005085529358No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTA3913.615349052242256No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC2432.246879334257975No Hit
CTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGG1921.7753120665742024No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTC1521.4054553860379104No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGTCGCC1261.1650485436893203Illumina Single End PCR Primer 1 (100% over 22bp)
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1010.9338881183541379No Hit
ATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGG670.6195099398982894No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT640.5917706888580675No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC640.5917706888580675No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGCCA630.5825242718446602No Hit
TCTTTATACTCCTCGGCTTCTTCCAAGTCTTTTTCACTTTCTAGTATCTG390.36061026352288483No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTG370.34211742949607027No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGTCGGC370.34211742949607027Illumina Single End PCR Primer 1 (95% over 22bp)
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370.34211742949607027No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGGCGCC340.31437817845584837Illumina Single End PCR Primer 1 (95% over 22bp)
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAG330.3051317614424411No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC310.28663892741562647No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAG280.2588996763754045No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG280.2588996763754045No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG280.2588996763754045No Hit
CTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGTTCTCGG260.24040684234858992No Hit
TTATTAAACACAGCAGTGGCTGAAAATAATGAATTGTAGAATGGACCCCA230.21266759130836801No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA210.1941747572815534No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA210.1941747572815534No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200.18492834026814609No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAAAA190.1756819232547388No Hit
GTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATC190.1756819232547388No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGGGGTCGCC190.1756819232547388Illumina Single End PCR Primer 1 (95% over 22bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC180.1664355062413315No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT180.1664355062413315No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG170.15718908922792418No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGTTCTC170.15718908922792418No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA160.14794267221451687No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA160.14794267221451687No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGGCGGC150.13869625520110956No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA150.13869625520110956No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC150.13869625520110956No Hit
CATTAAGTTATGCAAAGAAAAATAAATGCAACTGCATAAAAAGAACCACG140.12944983818770225No Hit
ATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGGGG140.12944983818770225No Hit
CTCCAAGATTCAAGTTTTCCAGTTTCAAAGGCAGTTCCGGGGTTGAGCCC140.12944983818770225No Hit
GGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCGTTATAGT130.12020342117429496No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC130.12020342117429496No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC130.12020342117429496No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAA120.11095700416088765No Hit
ATTAAACACAGCAGTGGCTGAAAATAATGAATTGTAGAATGGACCCCAGG120.11095700416088765No Hit
GCACTACACGGGCTTCTTTATACTCCTCGGCTTCTTCCAAGTCTTTTTCA110.10171058714748035No Hit
GTAGAATGGACCCCAGGATTTAATGCAAAAAACATCCCATACAGAAAAAT110.10171058714748035No Hit
CATTTATTTGACACATTAAGTTATGCAAAGAAAAATAAATGCAACTGCAT110.10171058714748035No Hit
ATCCAGCACTACACGGGCTTCTTTATACTCCTCGGCTTCTTCCAAGTCTT110.10171058714748035No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGA110.10171058714748035No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAATGTA110.10171058714748035No Hit
CTCTAGAAGAGGGTGTCAGATCTTGTTACAGATGGTTATGAACCACCATG110.10171058714748035No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGTCGCG110.10171058714748035Illumina Single End PCR Primer 1 (95% over 21bp)
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGGGGGCGCC110.10171058714748035No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.10171058714748035No Hit
GCCCTATACGTCGTCTTGAAGCCGACTTAGCAGAGCCCTGTGTTTTTGCT110.10171058714748035No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)