FastQCFastQC Report
Tue 7 May 2024
EGAF00007823073

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823073
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12143
Total Bases1.8 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC57

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGCAAGCTAATCG3032.49526476159104No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGCAAGCTAATCT2111.7376266161574572No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC1931.58939306596393No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1361.1199868236844273No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1311.0788108375195586No Hit
ATTGCGCAATGAAGGGCGAGGGCAAAGTCGTGCTATTAAAAGGTGGGTCT1100.9058716956271102No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGCAAGCT1010.8317549205303467No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCGGCAAGCTAATCG880.7246973565016882No Hit
ATTGCGCAATGGTATCTTCGGGATAAGAAAGACATCCTATGCGCAGCACC580.47764143951247634No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGCAAGCTAAT550.4529358478135551No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC490.4035246644157128No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGC460.37881907271679155No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG400.32940788931894915No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCGGCAAGCTAATCT370.304702297620028No Hit
ATTGCGCAATGAGGATGGGGGGGAAAAGTGATAAGTGAATATGATAAGAG330.2717615086881331No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC330.2717615086881331No Hit
ATTGCGCAATGTGGCGACGGGGGGGTTGTAAACCTCTTTCAGTAGGGAAG320.26352631145515937No Hit
ATTGCGCAATGCGCTGTACGGGGTTGTGGGTGAGTGAGTGATCCTGTACT290.23882071975623817No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT280.23058552252326445No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT280.23058552252326445No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT250.20587993082434325No Hit
ATTGCGCAATGGCTGTACGGGGTTGTGGGTGAGTGAGTGATCCTGTACTG250.20587993082434325No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC240.1976447335913695No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC230.18940953635839577No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA210.17293914189244833No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGCAAGCTAAGCG210.17293914189244833No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT200.16470394465947458No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG200.16470394465947458No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA190.15646874742650088No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGCAAGC180.14823355019352713No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC160.13176315572757968No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA160.13176315572757968No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT160.13176315572757968No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG160.13176315572757968No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT150.12352795849460595No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGG140.11529276126163222No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC140.11529276126163222No Hit
ATTGCGTAATGCCGCGCCAGGGGCTTCTTCTTCTTCGGGAGAACACCAAA140.11529276126163222No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG130.10705756402865849No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG130.10705756402865849No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)