FastQCFastQC Report
Tue 7 May 2024
EGAF00007823078

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823078
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9134
Total Bases1.3 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC53

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTA4244.641996934530327No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT3113.404860959054084No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1892.0691920297788484No Hit
CTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGG1391.521786730895555No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTC1331.45609809502956No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGTCGCC1041.1386030216772498Illumina Single End PCR Primer 1 (100% over 22bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT730.7992117363696081No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGCCA630.6897306765929494No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT620.6787825706152836No Hit
ATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGG510.5583534048609591No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGGCGCC380.41602802715130277Illumina Single End PCR Primer 1 (95% over 22bp)
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC380.41602802715130277No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTG370.40507992117363695No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAG330.3612874972629735No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG330.3612874972629735No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAG270.29559886139697833No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC260.28465075541931245No Hit
CTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGTTCTCGG250.27370264944164663No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG230.25180643748631487No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG230.25180643748631487No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGTCGGC230.25180643748631487Illumina Single End PCR Primer 1 (95% over 22bp)
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230.25180643748631487No Hit
GTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATC210.22991022553098314No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC200.21896211955331726No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG190.20801401357565139No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC190.20801401357565139No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGTGGCC180.19706590759798556Illumina Single End PCR Primer 1 (95% over 22bp)
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT170.18611780162031968No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.18611780162031968No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGA160.17516969564265383No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA160.17516969564265383No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC150.16422158966498795No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC150.16422158966498795No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAA140.15327348368732208No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA140.15327348368732208No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA140.15327348368732208No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA130.14232537770965623No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA120.13137727173199037No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGTTCTCGGTGGTCGCC120.13137727173199037Illumina Single End PCR Primer 1 (95% over 22bp)
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAA110.1204291657543245No Hit
TCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAG110.1204291657543245No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.1204291657543245No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGA100.10948105977665863No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA100.10948105977665863No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)