FastQCFastQC Report
Tue 7 May 2024
EGAF00007823086

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823086
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2303
Total Bases347.7 kbp
Sequences flagged as poor quality0
Sequence length151
%GC62

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT38416.673903603994788No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1556.730351715154146No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGTCGCC1144.950065132435953Illumina Single End PCR Primer 1 (100% over 22bp)
CTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGG893.864524533217542No Hit
ATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGG532.301346070343031No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTC512.214502822405558No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC502.1710811984368217No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGGCGCC361.5631784628745113Illumina Single End PCR Primer 1 (95% over 22bp)
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGTCGGC291.2592270950933566Illumina Single End PCR Primer 1 (95% over 22bp)
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTA220.9552757273122015No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGGGGTCGCC190.8250108554059922Illumina Single End PCR Primer 1 (95% over 22bp)
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.7381676074685193No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGTCGCG120.5210594876248371Illumina Single End PCR Primer 1 (95% over 21bp)
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGTGGCC120.5210594876248371Illumina Single End PCR Primer 1 (95% over 22bp)
CTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGTTCTCGG110.4776378636561007No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGGGGTCGGC100.43421623968736434No Hit
ATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGGGG100.43421623968736434No Hit
GTCTCTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATC100.43421623968736434No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGTTCTC80.34737299174989145No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGTTCTCGGTGGTCGCC70.303951367781155Illumina Single End PCR Primer 1 (95% over 22bp)
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGGCGGC70.303951367781155No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.303951367781155No Hit
ATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGTTCTCGGTGG70.303951367781155No Hit
GGATACCACGTGTCCCGCCCTACTCTTCGAGTTCACAACCTGTGTGTTTT60.26052974381241856No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGGGGTCGCG50.21710811984368217Illumina Single End PCR Primer 1 (95% over 21bp)
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGGGGGCGCC50.21710811984368217No Hit
TATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTG50.21710811984368217No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA50.21710811984368217No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGTGGGC50.21710811984368217No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50.21710811984368217No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.21710811984368217No Hit
CACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGTCG50.21710811984368217No Hit
ATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCG40.17368649587494572No Hit
ACCCTGTACTCTGCGACTTTCCAGACGCTTCCACTAACACACAAGCTGAT40.17368649587494572No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGGGGGC40.17368649587494572No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGTTCTCGGTGGTGGGC40.17368649587494572No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTATATCTCGGTGGTCGCC40.17368649587494572Illumina Single End PCR Primer 1 (95% over 22bp)
CTTATACACATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCGCGG40.17368649587494572No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.17368649587494572No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC40.17368649587494572No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC40.17368649587494572No Hit
GTATAAGAGACCGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC40.17368649587494572No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGGCGCG30.13026487190620928Illumina Single End PCR Primer 1 (95% over 21bp)
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGTTCTCGGTGGTCGGC30.13026487190620928No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACAGGAAGTGCCAGTGTAG30.13026487190620928No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG30.13026487190620928No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGGGGCC30.13026487190620928No Hit
CATCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGTGGCG30.13026487190620928Illumina Single End PCR Primer 1 (95% over 21bp)
GTGTATAAGAGACAGATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.13026487190620928No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAG30.13026487190620928No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATATTT30.13026487190620928No Hit
GTGTATAAGAGACAGAGTGCGCAATCTGTCTCTTATACACATCTGACGCT30.13026487190620928No Hit
TCTGACGCTGCCGACGAGGAAGTGCCAGTGTAGATCTCGGTGGTCGCCGT30.13026487190620928Illumina Single End PCR Primer 1 (100% over 24bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC30.13026487190620928No Hit
GTATCACCCTGTACTCTGCGACTTTCCAGACGCTTCCACTAACACACAAG30.13026487190620928No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC30.13026487190620928No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)