FastQCFastQC Report
Tue 7 May 2024
EGAF00007823089

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823089
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7877
Total Bases1.1 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC2723.4530912784054846No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1792.2724387457153736No Hit
ATTGCGCAATGGCTTTACGGGGGATGGTTGTCCGGGGCTAAGTGTGTGGA971.2314332867843087No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA941.19334772121366No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGATCTCT320.40624603275358645No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG270.34277009013583853No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGATCTCTCATCG250.31737971308873936No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC250.31737971308873936No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGA240.3046845245651898No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTAGATCTCTCATCG240.3046845245651898No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGATCTCTCAT220.2792941475180907No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG210.26659895899454106No Hit
ATTGCGCAATGCAGGCAACGGGGAACTTGGAGATAGCTGGTTCTCTCCGA210.26659895899454106No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA200.2539037704709915No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAG150.1904278278532436No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTAGGTCTCTCATCG140.17773263932969405No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA130.16503745080614446No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTAGTTCTCTCATCG130.16503745080614446No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGATCTCTCATCT130.16503745080614446No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGTCTCTCATCG120.1523422622825949No Hit
ATTGCGCAATGACGTGATGGGGCTGGCTCCGGTGCGCTTGTGACGGCCCG100.12695188523549575No Hit
GGGGGGTGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG90.11425669671194617No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC90.11425669671194617No Hit
GGGGGCTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG80.10156150818839661No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTAGATCTCTCATCT80.10156150818839661No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC80.10156150818839661No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG80.10156150818839661No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)