FastQCFastQC Report
Mon 16 Jan 2023
EGAF00007823102

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823102
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3608
Sequences flagged as poor quality0
Sequence length151
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT1845.099778270509978No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC992.7439024390243905No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGT611.6906873614190687No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.8037694013303769No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280.7760532150776054No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGG260.720620842572062No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATC190.5266075388026608No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC170.47117516629711753No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGTT170.47117516629711753No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGA150.41574279379157425No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAG140.3880266075388027No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG110.3048780487804878No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC110.3048780487804878No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG90.24944567627494454No Hit
CTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGGTGTGGG90.24944567627494454No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGC90.24944567627494454No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGC90.24944567627494454No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGTA90.24944567627494454No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC80.22172949002217296No Hit
CATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGTTGTGGGGGGGGGGG80.22172949002217296No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG80.22172949002217296No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.22172949002217296No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC80.22172949002217296No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGTG80.22172949002217296No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC70.19401330376940135No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA70.19401330376940135No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA70.19401330376940135No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTG70.19401330376940135No Hit
CATCTGACGCTGCCGACGAGGTGCGTATCGTGGGGGTGTGGGGGGGGGGG70.19401330376940135No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA60.1662971175166297No Hit
CTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGGGGTGTGGG60.1662971175166297No Hit
CTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGTTGTGGG60.1662971175166297No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA60.1662971175166297No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGGGGTGTC60.1662971175166297No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA60.1662971175166297No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC60.1662971175166297No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT50.1385809312638581No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC50.1385809312638581No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAAAA50.1385809312638581No Hit
CTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGGGTGGTGGG50.1385809312638581No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAAAA50.1385809312638581No Hit
CATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGGTGTGGGGGGGGGGG50.1385809312638581No Hit
CTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGTAGATCTCGG50.1385809312638581No Hit
CATCTGACGCTGCCGACGAGGTGCGTATCGTGTAGATCTCGGTGGTCGCC50.1385809312638581Illumina RNA PCR Primer (95% over 24bp)
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAA40.11086474501108648No Hit
ATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGGTGGGGGGGG40.11086474501108648No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCATTGCGCAA40.11086474501108648No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40.11086474501108648No Hit
ATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.11086474501108648No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAATGTA40.11086474501108648No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAA40.11086474501108648No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGTAGATCTC40.11086474501108648No Hit
ATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGGGGTGTGGGGGG40.11086474501108648No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAAA40.11086474501108648No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG40.11086474501108648No Hit
CTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGTGGGGGG40.11086474501108648No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGTTGTG40.11086474501108648No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGGTGGG40.11086474501108648No Hit
CTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGGGGTGGCGG40.11086474501108648No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAAAATTT40.11086474501108648No Hit
CATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGGTGTCGGGGGGGGGG40.11086474501108648No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA40.11086474501108648No Hit
CATCTGACGCTGCCGACGAGGTGCGTATCGTGGGGGTGTCGGGGGGGGGG40.11086474501108648No Hit
CTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGGGTTGTCGG40.11086474501108648No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAAA40.11086474501108648No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA40.11086474501108648No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA40.11086474501108648No Hit
TTTTTTTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACG40.11086474501108648No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)