FastQCFastQC Report
Mon 16 Jan 2023
EGAF00007823104

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823104
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4908
Sequences flagged as poor quality0
Sequence length151
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT2956.010594947025265No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1392.8321108394458028No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGT611.2428687856560716No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT380.7742461287693562No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC320.6519967400162999No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGG300.6112469437652812No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280.5704971475142625No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGC220.44824775876120615No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATC220.44824775876120615No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG150.3056234718826406No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGTA140.28524857375713125No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG120.24449877750611246No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.24449877750611246No Hit
CTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGGTGTGGG110.22412387938060307No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC110.22412387938060307No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAG110.22412387938060307No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC110.22412387938060307No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGA100.20374898125509372No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA100.20374898125509372No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA100.20374898125509372No Hit
CTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGGGGTGTGGG90.18337408312958436No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC90.18337408312958436No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC90.18337408312958436No Hit
CATCTGACGCTGCCGACGAGGTGCGTATCGTGTAGATCTCGGTGGTCGCC90.18337408312958436Illumina RNA PCR Primer (95% over 24bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG80.16299918500407498No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGTT80.16299918500407498No Hit
CTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGGGGTGTCGG80.16299918500407498No Hit
GGAATGTACAGTGCATATTGGCGGCGCTCGCCTCATTACGATTCGCCAGC80.16299918500407498No Hit
CATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGGTGTGGGGGGGGGGG80.16299918500407498No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAA70.14262428687856563No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAATGTAT70.14262428687856563No Hit
CATCTGACGCTGCCGACGAGGTGCGTATCGTGGGGGTGGGGGGGGGGGGG70.14262428687856563No Hit
CATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGTTGTGGGGGGGGGGG70.14262428687856563No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAA70.14262428687856563No Hit
CCTTAGGACCGTTATAGTTACGGCCGCCGTTTACTGGGGCTTCAATTCAA70.14262428687856563No Hit
CATCTGACGCTGCCGACGAGGTGCGTATCGTGGGGTTGTGGGGGGGGGGG70.14262428687856563No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGC70.14262428687856563No Hit
CTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGGGTTGTGGG70.14262428687856563No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGGGGTGTG70.14262428687856563No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAATTTA70.14262428687856563No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC70.14262428687856563No Hit
CTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGGTGTCGG70.14262428687856563No Hit
GTGCATATTGGCGGCGCTCGCCTCATTACGATTCGCCAGCTTGCTTCTCT60.12224938875305623No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGAAA60.12224938875305623No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG60.12224938875305623No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA60.12224938875305623No Hit
CTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGTTGGGGG60.12224938875305623No Hit
CCCTATACGTCGTCTTGCGACTTCGCAGAGCCCTGTGTTTTTGGTAAACA60.12224938875305623No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGTAGATCTC60.12224938875305623No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA60.12224938875305623No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTG60.12224938875305623No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.12224938875305623No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC60.12224938875305623No Hit
GTACAGTGCATATTGGCGGCGCTCGCCTCATTACGATTCGCCAGCTTGCT50.10187449062754686No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATAT50.10187449062754686No Hit
ATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGGGGTGTGGGGGG50.10187449062754686No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAAATTT50.10187449062754686No Hit
TCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA50.10187449062754686No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA50.10187449062754686No Hit
ATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGGGGTGGGGGGGG50.10187449062754686No Hit
GAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA50.10187449062754686No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGGGGTGGG50.10187449062754686No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC50.10187449062754686No Hit
AGGCTGTACTGATCATATTCATCCAGGATTCCATCTTCATCCTCTAGGTT50.10187449062754686No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGTTGTG50.10187449062754686No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)