FastQCFastQC Report
Tue 7 May 2024
EGAF00007823105

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823105
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9836
Total Bases1.4 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGCAAGCTAATCG2072.104514030093534No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC1891.9215128100854006No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGCAAGCTAATCT1141.159007726718178No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1031.0471736478243188No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCGGCAAGCTAATCG810.8235054900366002No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA770.782838552257015No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGCAAGCT620.6303375355835705No Hit
ATTGCGCAATGAGGTGGCCGGGTAAGTTATCCGCCGAAGCTGTGGCAACC460.4676697844652297No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGCAAGCTAAT450.4575030500203334No Hit
ATTGCGCAATGCGTTATCAGGGCGTTGGAAATTTGAGAAGGTCTGTCCTT390.39650264335095564No Hit
ATTGCGCAATGTGGGGAGAGGGAGACAAGGTCTGCAAGTACTTCTTGCAA370.3761691744611631No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGC350.35583570557137045No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG350.35583570557137045No Hit
ATTGCGCAATGTAAGGTAGGGGGGATGACTAAGGGTTCCTGGGTCAAGTT330.33550223668157786No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCGGCAAGCTAATCT310.31516876779178526No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA250.2541683611224075No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG250.2541683611224075No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT220.22366815778771856No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA220.22366815778771856No Hit
ATTGCGCAATGTCGCATATGGGGCGAGCGAACCGGGAAGAGCCCAGCTTG210.21350142334282227No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC210.21350142334282227No Hit
ATTGCGCAATGGAGCGAACGGGAAGGGTTCCTGGGTCAAGTTCGTCTTCC200.20333468889792597No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC200.20333468889792597No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT180.18300122000813338No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT180.18300122000813338No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC180.18300122000813338No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGG170.17283448556323708No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT170.17283448556323708No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGCAAGCTAAGCG170.17283448556323708No Hit
ATTGCGCAATGTACTCAACGGGAGAGGGTGAGAATCCCGTACTTGCCATG160.16266775111834078No Hit
ATTGCGCAATGATTATCGCGGGCGGCAACCGCCGGATCGCCGTGCACTCC150.15250101667344448No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGC150.15250101667344448No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG150.15250101667344448No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGCAAGCTCATCG130.1321675477836519No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG130.1321675477836519No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT130.1321675477836519No Hit
ATTGCGCAATGCAATTTCTGGGAGGAATAAACTAAAAATCGAGAGTTAAT130.1321675477836519No Hit
ATTGCGCAATGTGCTGGCTGGTGATGAATTCTCAGTTGCATATGACAGAA120.12200081333875558No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC120.12200081333875558No Hit
ATTGCGCAATGGCGGGGAGGGGGGGATGTGAGAAGAACGCACAAGAGATA120.12200081333875558No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC110.11183407889385928No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGCAAGC110.11183407889385928No Hit
ATCTGTGGCTTATACACATCTCCGAGCCCACGAGACCGGCAAGCTAATCG100.10166734444896298No Hit
ATTGCGCAATGACAGTCACGGGGAAGGGCACACAAAGGATGCCTTGGTAG100.10166734444896298No Hit
ATTGCGCAATGATCCTCGAGTGTGAGTTAAAATAAAGATTGCAGGGCCCC100.10166734444896298No Hit
ATTGCGCAATGACACACCAGGGGGGGGACCATCCTCCAAGGCTAAATACT100.10166734444896298No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC100.10166734444896298No Hit
ATTGCGCAATGGGGGCGGGGGAACAGTACTTTTGTTAGTTAGTCCCCCAC100.10166734444896298No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGCAAGCTAATGG100.10166734444896298No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA100.10166734444896298No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG100.10166734444896298No Hit
ATTGCGCAATGTAGCCTGTGGGGAACGTCTGCCCTATCAACTTTCGATGG100.10166734444896298No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)