FastQCFastQC Report
Tue 7 May 2024
EGAF00007823106

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823106
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9836
Total Bases1.4 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT2582.623017486783245No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGT2582.623017486783245No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGG1501.525010166734445No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1381.4030093533956893No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGTT930.9455063033753559No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT770.782838552257015No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATC740.7523383489223262No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGTA650.6608377389182595No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGA580.5896705978039853No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT540.5490036600244002No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTG540.5490036600244002No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGC430.43716958113054083No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC350.35583570557137045No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC350.35583570557137045No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG340.34566897112647416No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGTG330.33550223668157786No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA280.2846685644570964No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG280.2846685644570964No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC260.2643350955673038No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG250.2541683611224075No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA230.23383489223261486No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTG210.21350142334282227No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAG200.20333468889792597No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGC190.1931679544530297No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG180.18300122000813338No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC180.18300122000813338No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC180.18300122000813338No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.18300122000813338No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT170.17283448556323708No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.16266775111834078No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA140.1423342822285482No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA140.1423342822285482No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA130.1321675477836519No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGCGTAT130.1321675477836519No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA130.1321675477836519No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC120.12200081333875558No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGA110.11183407889385928No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA110.11183407889385928No Hit
CCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGC100.10166734444896298No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA100.10166734444896298No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC100.10166734444896298No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)