FastQCFastQC Report
Mon 16 Jan 2023
EGAF00007823116

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823116
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3339
Sequences flagged as poor quality0
Sequence length151
%GC59

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT2005.989817310572028No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC942.815214135968853No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC280.8385744234800839No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.5390835579514826No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.44923629829290207No Hit
CTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGGTGTGGG130.3893381251871818No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGT120.3593890386343217No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG100.2994908655286014No Hit
CTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGTAGATCTCGG100.2994908655286014No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC100.2994908655286014No Hit
CTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGGGGTGTCGG90.2695417789757413No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAG90.2695417789757413No Hit
CTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGGGGTGTGGG80.23959269242288112No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA80.23959269242288112No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC80.23959269242288112No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.23959269242288112No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG70.20964360587002098No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC70.20964360587002098No Hit
CTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGGGTTGGGGG70.20964360587002098No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATC70.20964360587002098No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA70.20964360587002098No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.20964360587002098No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGG70.20964360587002098No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG60.17969451931716085No Hit
CATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGTTGTGGGGGGGGGGG60.17969451931716085No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG60.17969451931716085No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGTTGTG60.17969451931716085No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA60.17969451931716085No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA60.17969451931716085No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGTA60.17969451931716085No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAA50.1497454327643007No Hit
CTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGGTGGGGG50.1497454327643007No Hit
GAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA50.1497454327643007No Hit
CATCTGACGCTGCCGACGAGGTGCGTATCGTGGGGGTGGGGGGGGGGGGG50.1497454327643007No Hit
TCACTAAATCGCAGGCTCATTCTTCAAAAGGCACGCCGTCACCACACAGG50.1497454327643007No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGTAGATCTC50.1497454327643007No Hit
ATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGGGGTGTGGGGGG50.1497454327643007No Hit
ATGCCACATCGAGCGAAGCACGCCATGGGCGATATTACTAGTTCTCACGC50.1497454327643007No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAATTTAA50.1497454327643007No Hit
CATCTGACGCTGCCGACGAGGTGCGTATCGTGGGGTTGTGGGGGGGGGGG50.1497454327643007No Hit
CTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGGGGTGGGGG50.1497454327643007No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTG50.1497454327643007No Hit
CTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGTTGGTGTGGG40.11979634621144056No Hit
CTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGGGTTGTGGG40.11979634621144056No Hit
ATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGGGGTGGGGGTGG40.11979634621144056No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT40.11979634621144056No Hit
ATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGGGGTGGGGGGGG40.11979634621144056No Hit
CTTATAGAACGTAGTCATTGGCTCATCAGCGGAGCGAATCTGCAGCTGCA40.11979634621144056No Hit
CATCTGACGCTGCCGACGAGGTGCGTATCGTGGGGGTGGGGGGGGGGGGC40.11979634621144056No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTG40.11979634621144056No Hit
CATCTGACGCTGCCGACGAGGTGCGTATCGTGGGGGTGGCGGGGGGGGGG40.11979634621144056No Hit
CATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGGGGTGGGGGGGGGGG40.11979634621144056No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGGGGTGTG40.11979634621144056No Hit
CATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGTTGGGGGGGGGGGGG40.11979634621144056No Hit
CTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGGTGTCGG40.11979634621144056No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGTTGGG40.11979634621144056No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCCCAA40.11979634621144056No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGGTGGG40.11979634621144056No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGC40.11979634621144056No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.11979634621144056No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCA40.11979634621144056No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGCGTAT40.11979634621144056No Hit
CTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGGTGTTGGCGG40.11979634621144056No Hit
CATCTGACGCTGCCGACGAGGTGCGTATCGTGGGGGTGTGGGGGGGGGGG40.11979634621144056No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGTGCGTATCGTGTT40.11979634621144056No Hit
CATCTGACGCTGCCGACGAGGTGCGTATCGTGTAGATCTCGGTGGTCGCC40.11979634621144056Illumina RNA PCR Primer (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)