FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007823125

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823125
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14357
Sequences flagged as poor quality0
Sequence length151
%GC55

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGACCATGAAATCG6954.840844187504353No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGACCATGAAATCT5393.754266211604096No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2611.817928536602354No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC1811.2607090617817092No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1521.0587170021592254No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGACCATGA850.5920456919969352No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGACCATGAAAT760.5293585010796127No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTGAC680.4736365535975482No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG660.4597060667270321No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC600.4179146061154837No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC550.38308838893919345No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT520.3621926586334192No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTGACCATGAAATCG450.31343595458661283No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGAC370.25771400710454834No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT350.24378352023403219No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC350.24378352023403219No Hit
ATTGCGCAATGTATACCGGGGGAGGGTTGCCAACCCGCGAGGGGGAGCTA310.21592254649299994No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT290.2019920596224838No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC290.2019920596224838No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA270.1880615727519677No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA260.1810963293167096No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTGACCATG250.17413108588145157No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT250.17413108588145157No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTGACCATGAAATCT230.16020059901093545No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC210.14627011214041932No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGACC210.14627011214041932No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG210.14627011214041932No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGACCATGAAAGCG200.13930486870516123No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG200.13930486870516123No Hit
ATTGCGCAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGACC190.13233962526990317No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT170.11840913839938705No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC170.11840913839938705No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG170.11840913839938705No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTCT160.111443894964129No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGACCATGAAAGCT150.10447865152887092No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT150.10447865152887092No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)