FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007823126

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823126
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14357
Sequences flagged as poor quality0
Sequence length151
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTATCTGGTGGG3752.6119662882217733No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTATCTGGTGGT3602.5074876366929026No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT2371.6507626941561608No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1911.33036149613429No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1461.0169255415476772No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTATCTGGTGTT1230.8567249425367417No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1010.7034895869610643No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTATCTGGTGTA880.6129414223027094No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTATCTG800.5572194748206449No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTT650.45274082329177406No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTATCTGGTGTG620.4318450929859999No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTATCTGGTG600.4179146061154837No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAC550.38308838893919345No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTATCTGGTGGA450.31343595458661283No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG440.3064707111513547No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG440.3064707111513547No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG410.2855749808455806No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA360.2507487636692902No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC360.2507487636692902No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT360.2507487636692902No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350.24378352023403219No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTT310.21592254649299994No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC300.20895730305774185No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC260.1810963293167096No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA250.17413108588145157No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTATCT230.16020059901093545No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTATCGGGTGGG230.16020059901093545No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG220.15323535557567736No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCGTATCTGGTGGT220.15323535557567736No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCGTATCTGGTGGG220.15323535557567736No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAA200.13930486870516123No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC200.13930486870516123No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200.13930486870516123No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTATCTGGGGGT180.1253743818346451No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTTTCTGGTGGG180.1253743818346451No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA170.11840913839938705No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC160.111443894964129No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA160.111443894964129No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC150.10447865152887092No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCT150.10447865152887092No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC150.10447865152887092No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGA150.10447865152887092No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG150.10447865152887092No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)