FastQCFastQC Report
Mon 16 Jan 2023
EGAF00007823131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7003
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGTGAAGCGATCG3004.283878337855205No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGTGAAGCGATCT2153.0701128087962304No Hit
ATTGCGCAATGAATCTAGGGGTGGGAAAGGATGTGAGGATCCCAAAACAA1952.784520919605883No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1582.2561759246037414No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC1141.627873768384978No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC761.0852491789233187No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG390.5569041839211766No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGTGAAGC370.5283449950021419No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC370.5283449950021419No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGTGAAGCGAT290.4141082393260031No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGT260.3712694559474511No Hit
ATTGCGCAATGCTTCCACCGGGGTGGAAGAGATAACCGCTGAAAGCATCT230.3284306725688991No Hit
ATTGCGCAATGGGTATTCTGGGGTCATTTCATTCCCTGTTAAAGGGGAAG220.3141510781093817No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT220.3141510781093817No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA190.2713122947308297No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT180.2570327002713123No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT170.24275310581179493No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA160.2284735113522776No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA160.2284735113522776No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC150.21419391689276027No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC120.1713551335142082No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGT120.1713551335142082No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG110.15707553905469085No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT110.15707553905469085No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC110.15707553905469085No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTCGTGAAGCGATCG110.15707553905469085No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT90.12851635013565615No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG90.12851635013565615No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGGTGCC90.12851635013565615No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT80.1142367556761388No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC80.1142367556761388No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC80.1142367556761388No Hit
ATTGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.1142367556761388No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC80.1142367556761388No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT80.1142367556761388No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)