FastQCFastQC Report
Tue 7 May 2024
EGAF00007823135

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823135
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12108
Total Bases1.8 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGATGGAGAAATCG4263.518334985133796No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGATGGAGAAATCT3693.047571853320119No Hit
ATTGCGCAATGGCGGAGACGGGGAGCATGCCGAGAAACTGATGAAGCTGC2361.949124545754873No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC2211.825239511067063No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2001.6518004625041296No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1621.3379583746283448No Hit
ATTGCGCAATGCGGGAGGAGGGGGAGGGCAAGATGAATGGGAAAGAAAAT1381.1397423191278493No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGATGGAGA840.6937561942517344No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG740.6111661711265279No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGATGGAGAAAT670.5533531549388834No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTGAT500.4129501156260324No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC470.38817310868847044No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC460.3799141063759498No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT450.3716551040634291No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA350.28906508093822264No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT330.27254707631318137No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT320.2642880740006607No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTGATGGAGAAATCG280.23125206475057813No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG260.2147340601255368No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA250.2064750578130162No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTGA240.19821605550049554No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC240.19821605550049554No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT230.1899570531879749No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGAT210.1734390485629336No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGATG200.16518004625041297No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA200.16518004625041297No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT200.16518004625041297No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC200.16518004625041297No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC200.16518004625041297No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG190.1569210439378923No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG180.14866204162537167No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGAGGGAGAAATCT180.14866204162537167No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG180.14866204162537167No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGAGGGAGAAATCG170.140403039312851No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTGATGGAG160.13214403700033034No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGATGGAGAAATGG160.13214403700033034No Hit
ATTGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.1238850346878097No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG150.1238850346878097No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT150.1238850346878097No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGG140.11562603237528907No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG140.11562603237528907No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTG130.1073670300627684No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTGATGGAGAAATCGCG130.1073670300627684No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)