FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007823139

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823139
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10741
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGCAATCCTCATCT3603.351643236197747No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGCAATCCTCATCG3503.258542035192254No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2212.0575365422213947No Hit
ATTGCGCAATGTAGAGGGAGGGGGAATCCGGGAGGACCATCTCCCAAGGC1641.5268596964900847No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC1221.1358346522670142No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC980.9123917698538311No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGCAATCCT790.7354994879433945No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGCAATCCTCAT590.5492970859324086No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG560.5213667256307606No Hit
ATTGCGCAATGCCGATATTGGGGGGGAGCCCATGGTTGTGGGTGAGTGAG560.5213667256307606No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGCA480.44688576482636627No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC410.38171492412252117No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT400.3724048040219719No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACGGCAATCCTCATCG390.3630946839214226No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT360.33516432361977466No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT360.33516432361977466No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC290.2699934829159296No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC290.2699934829159296No Hit
ATTGCGCAATGGCCGTGACGGGCGCTCTCTCCGCCCGAGCATCCCCACTC270.251373242714831No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG270.251373242714831No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACGGCAATCCTCATCT240.22344288241318314No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA240.22344288241318314No Hit
ATTGCGCAATGGTGGAGTGGGGAGAAGAGAAGGGCGCACGGTGAATGCCT230.21413276231263384No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGCAA220.20482264221208454No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG210.19551252211153525No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC200.18620240201098595No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT200.18620240201098595No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC190.17689228191043663No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG180.16758216180988733No Hit
ATTGCGCAATGGGCGGAACGGGGGCTCTCTCCGCCCGAGCATCCCCACTC180.16758216180988733No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGC170.15827204170933806No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG170.15827204170933806No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG170.15827204170933806No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC160.14896192160878877No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT160.14896192160878877No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTG150.13965180150823944No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTCT150.13965180150823944No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTG140.13034168140769015No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA140.13034168140769015No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT140.13034168140769015No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC140.13034168140769015No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT140.13034168140769015No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGC130.12103156130714085No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGCAATCCTCAGCG130.12103156130714085No Hit
ATTGCGCAATGCGGCAGTAGGGAGGGCTAGCCTCAAGTGATGATTATTGG130.12103156130714085No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT130.12103156130714085No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCCT130.12103156130714085No Hit
ATTGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.11172144120659157No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTGA120.11172144120659157No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTG120.11172144120659157No Hit
ATTGCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.10241132110604227No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTG110.10241132110604227No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT110.10241132110604227No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)