FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007823140

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823140
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10741
Sequences flagged as poor quality0
Sequence length151
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTATCTGGTGGG2262.104087142724141No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTATCTGGTGGT2152.001675821618099No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT1751.629271017596127No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1381.284796573875803No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1261.1730751326692113No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT880.8192905688483382No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTATCTGGTGTT680.6330881668373523No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTATCTG670.6237780467368029No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTATCTGGTGTG530.4934363653291128No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTATCTGGTG480.44688576482636627No Hit
CCTTCAACCTGGACATGGGTAGGTCACTTGGTTTCGGGTCTACGTCTACT420.3910250442230705No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTT420.3910250442230705No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTATCTGGTGTA410.38171492412252117No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT390.3630946839214226No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT380.35378456382087325No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA340.3165440834186761No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG330.3072339633181268No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTATCTGGTGGA320.29792384321757753No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAC310.2886137231170282No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC310.2886137231170282No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG290.2699934829159296No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC240.22344288241318314No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG220.20482264221208454No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG220.20482264221208454No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200.18620240201098595No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCGTATCTGGTGGT180.16758216180988733No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA180.16758216180988733No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT180.16758216180988733No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC170.15827204170933806No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA170.15827204170933806No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC170.15827204170933806No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA170.15827204170933806No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC170.15827204170933806No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAA160.14896192160878877No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC160.14896192160878877No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAG160.14896192160878877No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC160.14896192160878877No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA150.13965180150823944No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATA140.13034168140769015No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCT140.13034168140769015No Hit
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTA140.13034168140769015No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA130.12103156130714085No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG130.12103156130714085No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTTTCTGGTGGT130.12103156130714085No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTTTCTGGTGGG130.12103156130714085No Hit
GTATAGCCCTGGCTGTCCTGGAACTCACTTTGTAGACCAGGCTGGCCTCG130.12103156130714085No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCGTATCTGGTGGG120.11172144120659157No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCA120.11172144120659157No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA120.11172144120659157No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAA110.10241132110604227No Hit
ATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.10241132110604227No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC110.10241132110604227No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)