FastQCFastQC Report
Tue 7 May 2024
EGAF00007823143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10314
Total Bases1.5 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC55

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTACTAATCG4444.304828388598022No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTACTAATCT3673.5582703121970134No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2132.0651541593949974No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC1181.1440760131859609No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC830.8047314330036843No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTACT750.7271669575334496No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTACTAAT560.5429513282916424No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG540.5235602094240838No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTACG400.3878223773511732No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC380.3684312584836145No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT330.31995346131471786No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTACGCTACTAATCG270.2617801047120419No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT220.21330230754314522No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT210.2036067481093659No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC200.1939111886755866No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT180.17452006980802792No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC180.17452006980802792No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTACGCTACTAATCT170.16482451037424858No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGCT160.15512895094046927No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC160.15512895094046927No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC150.14543339150668994No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG150.14543339150668994No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC140.1357378320729106No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTACTAATGG140.1357378320729106No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT140.1357378320729106No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA130.1260422726391313No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG130.1260422726391313No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA130.1260422726391313No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTACTAAGCG130.1260422726391313No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTACTAATCGCG130.1260422726391313No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT120.11634671320535195No Hit
ATTGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.11634671320535195No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGC120.11634671320535195No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT120.11634671320535195No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG120.11634671320535195No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGC110.10665115377157261No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC110.10665115377157261No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG110.10665115377157261No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTAC110.10665115377157261No Hit
ATTGCGAAATGTGACAAATGGGGTTGGCGAGATCAACGAGTACGAGCAGG110.10665115377157261No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)