FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007823146

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823146
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14994
Sequences flagged as poor quality0
Sequence length151
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT3792.5276777377617714No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTATCTGGTGGG3582.387621715352808No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTATCTGGTGGT3542.3609443777511006No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC3012.007469654528478No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1761.1738028544751233No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1080.7202881152460985No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTATCTGGTGTT960.6402561024409764No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTATCTGGTGTA830.5535547552354275No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTATCTG790.5268774176337201No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTATCTGGTG770.5135387488328664No Hit
GCATACGACGAGCATCAGCAGCATACGACGAGCATCAGCAGCATACGACG700.4668534080298786No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTATCTGGTGTG690.4601840736294518No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAC640.42683740162731754No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTT530.3534747232226224No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530.3534747232226224No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC530.3534747232226224No Hit
CATCAGCAGCATACGACGAGCATCAGCAGCATACGACGAGCATCAGCAGC510.3401360544217687No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG430.286781379218354No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC390.26010404161664663No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG380.2534347072162198No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370.246765372815793No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG330.22008803521408565No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA320.21341870081365877No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTT320.21341870081365877No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTATCTGGTGGA310.20674936641323197No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC310.20674936641323197No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC300.20008003201280514No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG300.20008003201280514No Hit
AGCATCAGCAGCATACGACGAGCATCAGCAGCATACGACGAGCATCAGCA280.18674136321195145No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA260.1734026944110978No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA260.1734026944110978No Hit
GCAGCATACGACGAGCATCAGCAGCATACGACGAGCATCAGCAGCATACG240.1600640256102441No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC230.15339469120981725No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT230.15339469120981725No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC220.14672535680939042No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCGTATCTGGTGGG210.1400560224089636No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC200.13338668800853673No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.1267173536081099No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCGTATCTGGTGGT180.12004801920768307No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTATCT170.11337868480725624No Hit
GTGTGTACAAGGCCCGGGAACGTATTCACCGTGGCGTGCTGATCCACGAT160.10670935040682938No Hit
CTTATACACATCTGACGCTGCCGACGACGCTTATCTGGTGGGGGTGTGGG160.10670935040682938No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA160.10670935040682938No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAA150.10004001600640257No Hit
GAGTTCGGCGATCGACTCAAATCCACGGCCCACCGAGATATGCTCTTCCA150.10004001600640257No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTATCTGGGGGT150.10004001600640257No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTATCGGGTGGG150.10004001600640257No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTTTCTGGTGGG150.10004001600640257No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)