FastQCFastQC Report
Tue 7 May 2024
EGAF00007823147

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823147
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12541
Total Bases1.8 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCTCCAGAATCG5254.186269037556813No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCTCCAGAATCT4073.2453552348297583No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1931.538952236663743No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC1631.2997368630890678No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1551.2359460968024878No Hit
ATTGCGCAATGCACGTACCGGGCGGTGGCTCTGGTGGTGCCGCTGCTGGT890.7096722749382026No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCTCCAG890.7096722749382026No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCTCCAGAAT730.5820907423650427No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG700.5581692050075752No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC690.5501953592217527No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT680.5422215134359302No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGC520.41463998086277015No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT480.3827445977194801No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTAGCTCCAGAATCG400.31895383143290007No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT350.2790846025037876No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG330.2631369109321426No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC320.2551630651463201No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT310.24718921936049756No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC310.24718921936049756No Hit
ATTGCGCAATGCCACAACGGGGCGATTGCAGATGCAGAACAAGTACAGGT290.23124152778885254No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC290.23124152778885254No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC290.23124152778885254No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG270.21529383621720755No Hit
ATTGCGCAATGATCGTCCCGGGCTCTCCTACTTGGATAACTGTGGTAATT250.19934614464556258No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA250.19934614464556258No Hit
ATTGCGCAATGTTGGACCCGGGCTCTCCTACTTGGATAACTGTGGTAATT250.19934614464556258No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG240.19137229885974005No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCTCCAGAATCGCG220.17542460728809506No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAG220.17542460728809506No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC210.16745076150227253No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT210.16745076150227253No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT210.16745076150227253No Hit
ATTGCGCAATGCGCCGTGGGGGGGGCGTATGGCGGATGCCTAGGCTTTCA200.15947691571645004No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCTCCA200.15947691571645004No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTAGCTCCAGAATCT190.15150306993062754No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT190.15150306993062754No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG190.15150306993062754No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCTCCAGACTCG180.14352922414480504No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCT180.14352922414480504No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT170.13555537835898254No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGC170.13555537835898254No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGC170.13555537835898254No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTG160.12758153257316004No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA160.12758153257316004No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC160.12758153257316004No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGC150.11960768678733753No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT150.11960768678733753No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCTCCAGAAGCG140.11163384100151502No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCTCCAGAATCTCG130.10365999521569254No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG130.10365999521569254No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTCT130.10365999521569254No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT130.10365999521569254No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCT130.10365999521569254No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG130.10365999521569254No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)