FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007823148

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823148
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12541
Sequences flagged as poor quality0
Sequence length151
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTATCTGGTGGG3032.416075273104218No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTATCTGGTGGT2552.0333306753847378No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT2171.7303245355234829No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC2081.6585599234510806No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1000.7973845785822503No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT930.7415676580814927No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTATCTGGTGTT790.6299338170799776No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTATCTG730.5820907423650427No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTATCTGGTGTA700.5581692050075752No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTATCTGGTG560.4465353640060601No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTATCTGGTGTG470.3747707519336576No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG470.3747707519336576No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTT440.3508492145761901No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC390.3109799856470776No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAC340.2711107567179651No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT340.2711107567179651No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG310.24718921936049756No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTATCTGGTGGA300.23921537357467507No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250.19934614464556258No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG240.19137229885974005No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC240.19137229885974005No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG230.18339845307391756No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCGTATCTGGTGGT230.18339845307391756No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230.18339845307391756No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA220.17542460728809506No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA220.17542460728809506No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCT200.15947691571645004No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA190.15150306993062754No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTATCT190.15150306993062754No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTTTCTGGTGGG190.15150306993062754No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA180.14352922414480504No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTT180.14352922414480504No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTTTCTGGTGGT180.14352922414480504No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC170.13555537835898254No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC160.12758153257316004No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT160.12758153257316004No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC160.12758153257316004No Hit
CTTATACACATCTGACGCTGCCGACGACGCTTATCTGGTGGGGGTGTGGG150.11960768678733753No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTATCTGGGGGT150.11960768678733753No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC150.11960768678733753No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCGTATCTGGTGGG140.11163384100151502No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC140.11163384100151502No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA140.11163384100151502No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCA130.10365999521569254No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)