FastQCFastQC Report
Tue 17 Jan 2023
EGAF00007823151

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823151
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7843
Sequences flagged as poor quality0
Sequence length151
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTCTGCAAATCG2823.5955629223511414No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTCTGCAAATCT2272.8943006502613793No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1061.351523651663904No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC1041.3260232054060945No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC610.7777636108631901No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTCTGCAAAT470.5992604870585235No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG440.5610098176718092No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC380.4845084788983807No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTCTGCA360.4590080326405712No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGT310.3952569169960474No Hit
ATTGCGCAATGATTATGCGGGGATGTCATTGAGGCCCCAGATGTCAGCAC270.3442560244804284No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT260.33150580135152363No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTTGTCTGCAAATCG220.2805049088359046No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT200.2550044625780951No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC200.2550044625780951No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA180.2295040163202856No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT170.21675379319138086No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC170.21675379319138086No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC150.19125334693357135No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG150.19125334693357135No Hit
ATTGCGCAATGACGCACCGGGGGGTATTTATGACGAGACCGTTAGTAAAA150.19125334693357135No Hit
ATTGCGCAATGGTAGAGCGGGGGATCGAGTGAATCGTGGTGAAGGAACTC150.19125334693357135No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT150.19125334693357135No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT140.17850312380466657No Hit
ATTGCGCAATGACAATGGCGGGGACCTCCATTTAGGCAACTAGGTGGGGG140.17850312380466657No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG130.16575290067576182No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG130.16575290067576182No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC120.15300267754685706No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG110.1402524544179523No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTCTGCAAATGG100.12750223128904756No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTC100.12750223128904756No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTCTGCAAAGCG90.1147520081601428No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC90.1147520081601428No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG90.1147520081601428No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT90.1147520081601428No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGT90.1147520081601428No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA90.1147520081601428No Hit
ATTGCGCAATGCGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAG80.10200178503123804No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTTGTCTGCAAATCT80.10200178503123804No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTCTGCAAAGCT80.10200178503123804No Hit
ATTGCGCAATGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGT80.10200178503123804No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTCTGCAAATCTCG80.10200178503123804No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)