FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007823158

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823158
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12633
Sequences flagged as poor quality0
Sequence length151
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT5234.1399509221879205No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC3642.881342515633658No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAACGAATAGTGTA3322.6280376790944353No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAACGAATAGTGGT2812.224333095860049No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAACGAATAGTGTT2511.986859811604528No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAACGAATAGTGGA1871.4802501385260824No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT890.704504076624713No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAACGAATA800.6332620913480567No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAACGAATAGTG750.5936832106388031No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC580.45911501622734113No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAAC530.4195361355180876No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530.4195361355180876No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC380.3007994933903269No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG380.3007994933903269No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC350.2770521649647748No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG330.2612206126810734No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG310.245389060397372No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG280.22164173197181986No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAACGAATAGTGGG280.22164173197181986No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAACGAATAGTGTG260.2058101796881184No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA240.189978627404417No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA230.1820628512625663No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA220.1741470751207156No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.1741470751207156No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAACGAAT210.16623129897886488No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCA190.15039974669516346No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC190.15039974669516346No Hit
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAACG180.14248397055331277No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC180.14248397055331277No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAA180.14248397055331277No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA170.13456819441146206No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC170.13456819441146206No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAA160.12665241826961135No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAC160.12665241826961135No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC160.12665241826961135No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAAC150.11873664212776064No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA150.11873664212776064No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA150.11873664212776064No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT130.1029050898440592No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG130.1029050898440592No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.1029050898440592No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)